BME103:T130 Group 3 l2: Difference between revisions
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==Protocols== | ==Protocols== | ||
<font size=3><b>Materialsl</b></font><br><br> | |||
<font size=3><b>PCR Protocol</b></font><br><br> | <font size=3><b>PCR Protocol</b></font><br><br> | ||
1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | 1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | ||
2.) They were then cooled to fifty-seven degrees Celsius (57°C) for ten (10) seconds and the primers were attached to their matching sequences. <br> | 2.) They were then cooled to fifty-seven degrees Celsius (57°C) for ten (10) seconds and the primers were attached to their matching sequences. <br> | ||
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[[Image:Flourimeter Group 3.jpg|600x300px]]<br><br> | [[Image:Flourimeter Group 3.jpg|600x300px]]<br><br> | ||
<font size=3>'''DNA | <font size=3>'''DNA Measurement Protocol'''</font><br><br> | ||
<b>'''Fluorimeter Setup'''</b><br> | <b>'''Fluorimeter Setup'''</b><br> |
Revision as of 15:19, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterialsl PCR Protocol 1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. GoTaq Mix Components For 100μl reaction volume:
DNA Measurement Protocol Fluorimeter Setup Fluorimeter Measurements ImageJ Instructions Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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