BME103:T130 Group 3 l2: Difference between revisions
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PCR is a method of amplifying a sample of DNA. PCR alters the temperature and allows the DNA to separate, bind to primers, and catalyze. This results in the amount of DNA doubling after each cycle. <br> | PCR is a method of amplifying a sample of DNA. PCR alters the temperature and allows the DNA to separate, bind to primers, and catalyze. This results in the amount of DNA doubling after each cycle. <br> | ||
<b>Procedure:</b><br> | <b>Procedure:</b><br> | ||
1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for | 1.) The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | ||
2.) They were then cooled to fifty-seven degrees Celsius (57°C) and the primers were attached to their matching sequences. <br> | 2.) They were then cooled to fifty-seven degrees Celsius (57°C) and the primers were attached to their matching sequences. <br> | ||
3.) They were then heated back to seventy-two degrees Celsius (72°C) and polymerase extended the DNA strands by attaching the correct free nucleotides in order on the single strands. <br><br> | 3.) They were then heated back to seventy-two degrees Celsius (72°C) and polymerase extended the DNA strands by attaching the correct free nucleotides in order on the single strands. <br><br> |
Revision as of 15:01, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsPolymerase Chain Reaction GoTaq Mix Components For 100μl reaction volume:
Patient 2 Fluorimeter Setup Fluorimeter Measurements
ImageJ Instructions Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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