BME103:T130 Group 3 l2: Difference between revisions
Line 72: | Line 72: | ||
1.) Mix 0.1μL of template DNA, 0.5μL of 10μM forward primer, 0.5μL of 10μM reverse primer, 25.0μL of GoTaq Master Mix, and 23.9μL of dH20. All of these items mixed together should create a total volume of 50.0μL.<br> | 1.) Mix 0.1μL of template DNA, 0.5μL of 10μM forward primer, 0.5μL of 10μM reverse primer, 25.0μL of GoTaq Master Mix, and 23.9μL of dH20. All of these items mixed together should create a total volume of 50.0μL.<br> | ||
2.The DNA samples | 2.) Up to 16 of these samples are then loaded into the PCR machine<br> | ||
3.) The DNA samples are then heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | |||
4.) They are then cooled to fifty-seven degrees Celsius (57°C) for ten (10) seconds and the primers were attached to their matching sequences. <br> | |||
5.) Finally they are heated back to seventy-two degrees Celsius (72°C) for ten (10) seconds and polymerase extended the DNA strands by attaching the correct free nucleotides in order on the single strands. <br><br> | |||
<font size=3>'''DNA Measurement Protocol'''</font><br><br> | <font size=3>'''DNA Measurement Protocol'''</font><br><br> |
Revision as of 15:46, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol 1.) Mix 0.1μL of template DNA, 0.5μL of 10μM forward primer, 0.5μL of 10μM reverse primer, 25.0μL of GoTaq Master Mix, and 23.9μL of dH20. All of these items mixed together should create a total volume of 50.0μL. DNA Measurement Protocol Fluorimeter Setup Fluorimeter Measurements ImageJ Instructions Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
|