BME103:T130 Group 3 l2: Difference between revisions
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<font size=3><b>PCR Protocol</b></font><br><br> | <font size=3><b>PCR Protocol</b></font><br><br> | ||
1.) Mix 0.1μL of template DNA, 0.5μL of 10μM | 1.) Mix 0.1μL of template DNA, 0.5μL of 10μM forward primer, 0.5μL of 10μM reverse primer, 25.0μL of GoTaq Master Mix, and 23.9μL of dH20. All of these items mixed together should create a total volume of 50.0μL. | ||
2.The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | 2.The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. <br> | ||
3.) They were then cooled to fifty-seven degrees Celsius (57°C) for ten (10) seconds and the primers were attached to their matching sequences. <br> | 3.) They were then cooled to fifty-seven degrees Celsius (57°C) for ten (10) seconds and the primers were attached to their matching sequences. <br> |
Revision as of 15:41, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol 1.) Mix 0.1μL of template DNA, 0.5μL of 10μM forward primer, 0.5μL of 10μM reverse primer, 25.0μL of GoTaq Master Mix, and 23.9μL of dH20. All of these items mixed together should create a total volume of 50.0μL.
2.The DNA samples were heated to ninety-five degrees Celsius (95°C) for one (1) minute to unzip the two single strands. DNA Measurement Protocol Fluorimeter Setup Fluorimeter Measurements ImageJ Instructions Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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