BME103:T130 Group 3

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Protocols)
(OUR TEAM)
Line 14: Line 14:
|- valign="top"
|- valign="top"
| [[Image:Bunny.jpg|100px|thumb|Name: Serena Kaplan<br>Research and Development]]
| [[Image:Bunny.jpg|100px|thumb|Name: Serena Kaplan<br>Research and Development]]
-
| [[Image:Grumpy_cat.jpg|100px|thumb|Name: Gabe McInnis<br>Open PCR Machine Engineer]]
+
| [[Image:Pony.jpg|100px|thumb|Name: Gabe McInnis<br>Open PCR Machine Engineer]]
| [[Image:Puppy.jpg|100px|thumb|Name: Blake Eichler<br>Experimental Protocol Planner]]
| [[Image:Puppy.jpg|100px|thumb|Name: Blake Eichler<br>Experimental Protocol Planner]]
| [[Image:Giraffe.jpg|100px|thumb|Name: Sierra Morris<br>Experimental Protocol Planner]]
| [[Image:Giraffe.jpg|100px|thumb|Name: Sierra Morris<br>Experimental Protocol Planner]]

Revision as of 17:11, 8 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: Serena KaplanResearch and Development
Name: Serena Kaplan
Research and Development
Name: Gabe McInnisOpen PCR Machine Engineer
Name: Gabe McInnis
Open PCR Machine Engineer
Name: Blake EichlerExperimental Protocol Planner
Name: Blake Eichler
Experimental Protocol Planner
Name: Sierra MorrisExperimental Protocol Planner
Name: Sierra Morris
Experimental Protocol Planner
Name: Zazu MoloiOpen PCR Machine Engineer
Name: Zazu Moloi
Open PCR Machine Engineer
Name: Katelin VaughnResearch and Development
Name: Katelin Vaughn
Research and Development

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design



A Polymerase Chain Reaction (PCR) Machine (in the above image) is used to create large quantities of specific DNA sequences. This process consists of various heating and cooling cycles to unzip DNA strands and isolate the wanted DNA strands.

Experimenting With the Connections


When the LCD screen is disconnected from the open PCR circuit board, the LCD screen is shut off. The circuit board provides the power and input signals for the LCD screen, therefore, when the two parts are not connected the LCD screen will not function. When the 16-tube PCR block is disconnected from the PCR circuit board the block will not heat or cool. The fan and lid heater are both connected to the PCR circuit board with wires, so if this connection is disrupted, those parts will not function.

Test Run

(Write the date you first tested Open PCR and your experience(s) with the machine)




Protocols

Polymerase Chain Reaction
The DNA samples were heated to ninety-five degrees Celsius for three minutes to unzip the two single strands. They were then cooled to fifty-seven degrees Celsius and the primers were attached to their matching sequence. It was heated back to seventy-two degrees Celsius and polymerase extends DNA strands by attaching correct free nucleotides in order on the single strands.

Reagent Volume
Template DNA (20 ng)0.1μL
10μM forward primer0.5μL
10μM reverse primer0.5μL
GoTaq master mix25.0μL
dH2O23.9μL
Total Volume50.0μL


Patient 1
ID 30269
Male, 55 years old

Patient 2
ID 22057
Female, 55 years old


Flourimeter Measurements


(Add your work from Week 3, Part 2 here)




Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)




Results

(Your group will add the results of your Fluorimeter measurements from Week 4 here)


Personal tools