BME103:T130 Group 2 l2: Difference between revisions
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'''Background on Disease Markers''' | '''Background on Disease Markers'''<br> | ||
Transient Myeloproliferative Disorder of Down Syndrome is a combination of the diseases leukemia, down syndrome and acute myeloid. It is extremely rare and takes the life of a patient in the very early months of life. It is associated with the SNP (single nucleotide polymorphism) 121912500 on chromosome 21.<br> | '''Transient Myeloproliferative Disorder of Down Syndrome''' is a combination of the diseases leukemia, down syndrome and acute myeloid. It is extremely rare and takes the life of a patient in the very early months of life. It is associated with the SNP (single nucleotide polymorphism) 121912500 on chromosome 21.<br> | ||
http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=121912500 | http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=121912500 | ||
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'''Primer Design'''<br>< | '''Primer Design'''<br>< | ||
'''Transient Myeloproliferative Diorder of Down Syndrome:''' The forward primer of this disease is ACCGGCTG'''G'''TGGGCCCGCTG. The reverse primer that would correctly match the forward primer and not code for a diseased patient would be TGGCCGAC'''C'''ACCCGGGCGAC. The diseased allele and diseased patient will have a reverse primer TGGCCGAC'''A'''ACCCGGGCGAC. Therefore, a patient that contains the disease has an allele mutation within the pairing of G to C, it is instead G to A. This will give a positive on the PCR machine because it becomes single stranded due to the fact it cannot replicate properly. This, when we apply the SYBR green it will attach to only the single primers and the diseased patients will glow green. The replicated, normal strands, will appear clear when the SYBR green in added because it will not be able to attach to the replicated strands. | '''Transient Myeloproliferative Diorder of Down Syndrome:''' The forward primer of this disease is ACCGGCTG'''G'''TGGGCCCGCTG. The reverse primer that would correctly match the forward primer and not code for a diseased patient would be TGGCCGAC'''C'''ACCCGGGCGAC. The diseased allele and diseased patient will have a reverse primer TGGCCGAC'''A'''ACCCGGGCGAC. Therefore, a patient that contains the disease has an allele mutation within the pairing of G to C, it is instead G to A. This will give a positive on the PCR machine because it becomes single stranded due to the fact it cannot replicate properly. This, when we apply the SYBR green it will attach to only the single primers and the diseased patients will glow green. The replicated, normal strands, will appear clear when the SYBR green in added because it will not be able to attach to the replicated strands. | ||
Revision as of 10:54, 25 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features A. B. Glass oven like top C. double layered to fit twice the amount of samples New area for samples opens like an oven where the samples can be slid in. It will hold 32 samples instead of its original, 16. The sample trays will be placed one on top of an other when slid in. The glass is for seeing how tightly closed the lid is placed. Instructions
ProtocolsMaterials
PCR Protocol
Setting up the smart phone camera
Set the smart phone camera menu to the following:
1. Inactivate the flash.
DNA Measurement Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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