BME103:T130 Group 2 l2: Difference between revisions
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6. Place two drops of first diluted sample on top of the two drops of SYBR Green.<br> | 6. Place two drops of first diluted sample on top of the two drops of SYBR Green.<br> | ||
7. Turn on light and adjust glass slide so light goes directly through the drop.<br> | 7. Turn on light and adjust glass slide so light goes directly through the drop.<br> | ||
8. With equipment in the fluorimeter, Smartphone Operator will take pictures focused on the drop.<br> | 8. With equipment in the fluorimeter, Smartphone Operator will take pictures focused on the drop and give to image processor.<br> | ||
9. Using previously labeled waste pipette, remove drop from glass slide and discard waste.<br> | 9. Using previously labeled waste pipette, remove drop from glass slide and discard waste.<br> | ||
10. Repeat steps 5 through 9 for each sample, using next two consecutive, centered spots on glass slide for next sample. | 10. Repeat steps 5 through 9 for each sample, using next two consecutive, centered spots on glass slide for next sample. | ||
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'''Image Processing''' | |||
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1. Upload photo to ImageJ.<br> | |||
2. Click on ANALYZE, choose SET MEASUREMENTS, then AREA INTEGRATED DENSITY and MEAN GREY VALUE.<br> | |||
3. Click IMAGE, choose COLOR then SPLIT CHANNELS to create three files.<br> | |||
4. Choose image name that has "green" next to it.<br> | |||
5. Active Oval Selection by clicking on Menu bar.<br> | |||
6. Click and stretch oval around green or clear drop in image. Click ANALYZE and choose MEASURE.<br> | |||
7. Record sample number and measurements.<br> | |||
8. Draw another oval of the same size in the "green" file for the background above the drop to get the “noise”. Again, click ANALYZE and choose MEASURE.<br> | |||
9. Record sample name and measurements and use as background label. | |||
10. Save measurements to Excel file. | |||
==Research and Development== | ==Research and Development== |
Revision as of 15:36, 15 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
PCR Protocol
Setting up the smart phone camera
Set the smart phone camera menu to the following:
1. Inactivate the flash.
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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