BME103:T130 Group 15 l2: Difference between revisions
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==Protocols== | ==Protocols== | ||
'''Polymerase Chain Reaction'''<br> | '''Polymerase Chain Reaction'''<br> | ||
Polymerase chain reaction is basically molecular photocopying and the process or technique used to make copies of small segments of DNA because it only targets specific segments of the DNA and that's what makes it useful. PCR works by mixing two DNA fragments, also known as primers which are about 20 bases long. The mixture is then heated and denatured and then the primers bind to their complementary sequences on the separated strands. Then, the polymerase extends primers into new complementary strands and it goes through about 30 cycles. PCR products are useful and can be used in many experiments like DNA fingerprinting and detection of viruses. | Polymerase chain reaction is basically molecular photocopying and the process or technique used to make copies of small segments of DNA because it only targets specific segments of the DNA and that's what makes it useful. PCR works by mixing two DNA fragments, also known as primers which are about 20 bases long. The mixture is then heated and denatured and then the primers bind to their complementary sequences on the separated strands. Then, the polymerase extends primers into new complementary strands and it goes through about 30 cycles. PCR products are useful and can be used in many experiments like DNA fingerprinting and detection of viruses. | ||
# (Jim Dorsey) Polymerase Chain Reaction. http://www.contexo.info/DNA_Basics/polymerase_chain_reaction.htm. Last accessed 11/01/12.)<br> | # (Jim Dorsey) Polymerase Chain Reaction. http://www.contexo.info/DNA_Basics/polymerase_chain_reaction.htm. Last accessed 11/01/12.)<br> | ||
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<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method | |||
Things to consider: | |||
How should the PCR machine be set up? Does it need to be plugged in? | |||
How many samples will fit into a single 2-hour run? | |||
How many replicates should be created per patient? | |||
What should the final volume of the reaction be? | |||
Will signal reading be integrated into the PCR machine or remain separate? | |||
If it is separate, you will need to include instructions on how to use the fluorimeter. | |||
How should the user calculate the about of signal amplified? | |||
etc. | |||
---> | |||
'''Materials''' | |||
'''Supplied in Kit'''<br> | |||
{| border="1" class="wikitable" | |||
|+ Table 1 | |||
! PCR Machine | |||
! 1 | |||
|- | |||
| Instruction Manual | |||
| 1 | |||
|- | |||
| Extra screws/bolts/nuts | |||
| 5 each | |||
|- | |||
| USB Cable | |||
| 1 | |||
|- | |||
| Extra Battery | |||
| 1 | |||
|- | |||
| Power Cord | |||
| 1 | |||
|- | |||
|} | |||
<br> | |||
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here ---> | |||
'''PCR Protocol''' | |||
<br> | <br> | ||
'''Steps to Amplify DNA Samples'''<br> | '''Steps to Amplify DNA Samples'''<br> | ||
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<br> | <br> | ||
Revision as of 00:23, 26 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsPolymerase Chain Reaction
The Components of the GoTaq® Colorless Master Mix
DNA Samples (8)
DNA Measurement Protocol Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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