BME103:T130 Group 14: Difference between revisions
Hanna Rahman (talk | contribs) No edit summary |
Hanna Rahman (talk | contribs) No edit summary |
||
Line 56: | Line 56: | ||
<br> | <br> | ||
<br> | <br> | ||
#Acquire the DNA samples that have been submitted for testing <br> | |||
#Run the DNA samples through the thermal cycler program. <br> | |||
##Stage 1 (Initiation): 1 cycle, 95°C for 3 minutes. <br> | |||
##Stage 2 (Denaturation): 35 cycles, 95°C for 30 seconds, 57°C for 30 seconds, 72°C for 30 seconds.<br> | |||
##Stage 3 (Elongation) : 72°C for 3 minutes. <br> | |||
##Stage 4: Final hold for 4°C. <br> | |||
#After the DNA has been through the thermal cycle, mix each DNA sample with the PCR reaction mix (Taq DNA polymerase, MgCl2, dNTP’s, and a forward and reverse primer), using a separate pipette each time to reduce cross-contamination into 8 separate tubes. | |||
<br> | <br> | ||
<br> | <br> | ||
Line 88: | Line 88: | ||
---- | ---- | ||
|- | |- | ||
| GoTaq Master Mix | | GoTaq Master Mix | ||
---- | ---- | ||
| 50.0 microliters | | 50.0 microliters | ||
---- | ---- |
Revision as of 16:08, 1 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsPolymerase Chain Reaction The polymerase chain reaction (PCR) is biochemical machinery in biological chemistry to produce numerous copies of a particular piece of DNA, generating multiple duplicates of DNA sequences. In order to complete the reaction several components are required such as:
(Add your work from Week 3, Part 2 here)
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results(Your group will add the results of your Fluorimeter measurements from Week 4 here)
|