BME103:T130 Group 12 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: James Open PCR machine engineer Name: Heidi Hall Open PCR machine engineer Name: Justin Landstrom Student Protocol Planner Name: Chaio Protocol Planner Name: Jake R&D scientist LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design The two parts below that will be removed are the the knob and the bolt attached to it. These parts are present on the lid as a tightening mechanism but will be removed to improve the design. On the side of the picture are the two heating plates present in the lid. To compensate for removing the tightening mechanism, the plates will be lowered to make contact with the samples in the heating block at the point the lid snaps into place. Below is the top part of the general body of the PCR machine. It will be altered so buttons can be placed next to the LED screen. Key Features Instructions Protocols Materials Supplied in the Kit Amount PCR Machine (assembled) 1 Power Cord 1 Flourimeter Box 1 Flourimeter 1 Hydrophobic Slides 5 Supplied by User Amount Camera Phone 1 DNA Samples & Target Mix As many as desired Bulb pipettes As many as desired SYBR Green As much as desired Computer w/ ImageJ Software 1 PCR Protocol DNA Measurement Protocol 1. One pipette was used to transfer each sample into an Eppendorf tube that contained 400ml of buffer. The number of the sample used was labeled clearly and all of the sample was transferred into this Eppendorf tube. 2. Two drops of SYBR Green I was placed on the first two center drops. 3. Next, two drops of the diluted sample was added on top of the SYBR Green I solution drops. 4. The drop was then aligned with the light on the flourimeter. 5. A smartphone placed on a stand was used to take as many pictures as needed of the sample and that picture was sent to a computer for analysis. Research and Development Background on Disease Markers HIV is a virus that causes A.I.D.S. (acquired immunodeficiency syndrome). HIV affects the immune system by attacking specific cells that help the immune system work. These include the T Cells that help the white blood cells fend off bacteria, disease, and viruses. A SNP related HIV is rs1024611 and it affects Chromosome 17. Primer Design Illustration