BME103:T130 Group 12 l2

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(OUR TEAM)
(Research and Development)
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'''Background on Disease Markers'''
'''Background on Disease Markers'''
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<HIV is a virus that causes A.I.D.S. (acquired immunodeficiency syndrome).  HIV affects the immune system by attacking specific cells that help tthe immune system work.  These include the T Cells that help the white blood cells fend off bacteria, disease, and viruses.  A SNP related HIV is rs1024611 and it affects Chromosome 17.>
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<HIV is a virus that causes A.I.D.S. (acquired immunodeficiency syndrome).  HIV affects the immune system by attacking specific cells that help the immune system work.  These include the T Cells that help the white blood cells fend off bacteria, disease, and viruses.  A SNP related HIV is rs1024611 and it affects Chromosome 17.>

Revision as of 18:35, 15 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

Name: JamesOpen PCR machine engineer
Name: James
Open PCR machine engineer
Name: Heidi HallOpen PCR machine engineer
Name: Heidi Hall
Open PCR machine engineer
Name: Justin Landstrom StudentProtocol Planner
Name: Justin Landstrom Student
Protocol Planner
Name: ChaioProtocol Planner
Name: Chaio
Protocol Planner
Name: JakeR&D scientist
Name: Jake
R&D scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design
The two parts below that will be removed are the the knob and the bolt attached to it. These parts are present on the lid as a tightening mechanism but will be removed to improve the design. On the side of the picture are the two heating plates present in the lid. To compensate for removing the tightening mechanism, the plates will be lowered to make contact with the samples in the heating block at the point the lid snaps into place. Image:group12pic1.PNG

Below is the top part of the general body of the PCR machine. It will be altered so buttons can be placed next to the LED screen.

Image:group12pic2.PNG

Key Features


Instructions





Protocols

Materials


Supplied in the Kit Amount
PCR Machine (assembled) 1
Power Cord1
Flourimeter Box1
Flourimeter1
Hydrophobic Slides5


Supplied by User Amount
Camera Phone 1
DNA Samples & Target MixAs many as desired
Bulb pipettesAs many as desired
SYBR GreenAs much as desired
Computer w/ ImageJ Software1



PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers

<HIV is a virus that causes A.I.D.S. (acquired immunodeficiency syndrome). HIV affects the immune system by attacking specific cells that help the immune system work. These include the T Cells that help the white blood cells fend off bacteria, disease, and viruses. A SNP related HIV is rs1024611 and it affects Chromosome 17.>



Primer Design



Illustration


Personal tools