BME103:T130 Group 12 l2

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'''Components of PCR Master Mix'''
 
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| Camera Phone|| 1
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| DNA Samples & Target Mix||As many as desired
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| Bulb pipettes||As many as desired
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| SYBR Green||As much as desired
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| Computer w/ ImageJ Software||1
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Revision as of 18:33, 15 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: JamesOpen PCR machine engineer
Name: James
Open PCR machine engineer
Name: Heidi HallOpen PCR machine engineer
Name: Heidi Hall
Open PCR machine engineer
Name: Justin Landstrom StudentProtocol Planner
Name: Justin Landstrom Student
Protocol Planner
Name: ChaioProtocol Planner
Name: Chaio
Protocol Planner
Name: JakeR&D scientist
Name: Jake
R&D scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design
The two parts below that will be removed are the the knob and the bolt attached to it. These parts are present on the lid as a tightening mechanism but will be removed to improve the design. On the side of the picture are the two heating plates present in the lid. To compensate for removing the tightening mechanism, the plates will be lowered to make contact with the samples in the heating block at the point the lid snaps into place. Image:group12pic1.PNG

Below is the top part of the general body of the PCR machine. It will be altered so buttons can be placed next to the LED screen.

Image:group12pic2.PNG

Key Features


Instructions





Protocols

Materials


Supplied in the Kit Amount
PCR Machine (assembled) 1
Power Cord1
Flourimeter Box1
Flourimeter1
Hydrophobic Slides5


Supplied by User Amount
Camera Phone 1
DNA Samples & Target MixAs many as desired
Bulb pipettesAs many as desired
SYBR GreenAs much as desired
Computer w/ ImageJ Software1



PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


Personal tools