BME103:T130 Group 12 - Revision history

James Kyeh: /* Results */

James Kyeh — Thu, 15 Nov 2012 20:39:31 GMT

Results

←Older revision		Revision as of 20:39, 15 November 2012
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	==Results==		==Results==
		+	Below are the pictures that were put into ImageJ and used to gather data.
	[[Image:bme103pics.jpg]]		[[Image:bme103pics.jpg]]

Justin Landstrom at 20:39, 15 November 2012

Justin Landstrom — Thu, 15 Nov 2012 20:39:14 GMT

←Older revision		Revision as of 20:39, 15 November 2012
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	\|- valign="top"		\|- valign="top"
	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
-	\| [[Image:~~BME_103_Chiao~~.~~jpg~~\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]	+	\| [[Image:Frontpagepicture.JPG\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
	\| [[Image:jameskyeh.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]		\| [[Image:jameskyeh.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]

James Kyeh at 20:31, 15 November 2012

James Kyeh — Thu, 15 Nov 2012 20:31:56 GMT

←Older revision		Revision as of 20:31, 15 November 2012
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	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
	\| [[Image:BME_103_Chiao.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]		\| [[Image:BME_103_Chiao.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
-	\| [[Image:~~BME_103_Student~~.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]	+	\| [[Image:jameskyeh.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]
	\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]		\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]

Chiao May Lee: /* OUR TEAM */

Chiao May Lee — Thu, 15 Nov 2012 20:13:53 GMT

OUR TEAM

←Older revision		Revision as of 20:13, 15 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->

-	~~=OUR TEAM=~~	+

	{\| style="wikitable" width="700px"		{\| style="wikitable" width="700px"
	\|- valign="top"		\|- valign="top"
	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
-	\| [[Image:~~BME_103_Student~~.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]	+	\| [[Image:BME_103_Chiao.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
	\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]		\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]

Heidi Hall: /* Results */

Heidi Hall — Thu, 15 Nov 2012 16:12:08 GMT

Results

←Older revision		Revision as of 16:12, 15 November 2012
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	==Results==		==Results==
	[[Image:bme103pics.jpg]]		[[Image:bme103pics.jpg]]
-	~~<!--- Place two small Image J data images here. One showing the result of Water and the other showing the result of Calf Thymus DNA --->~~

	{\| {{table}}		{\| {{table}}

James Kyeh: /* Results */

James Kyeh — Thu, 15 Nov 2012 09:22:33 GMT

Results

←Older revision		Revision as of 09:22, 15 November 2012
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	==Results==		==Results==
-		+	[[Image:bme103pics.jpg]]
-		+
	<!--- Place two small Image J data images here. One showing the result of Water and the other showing the result of Calf Thymus DNA --->		<!--- Place two small Image J data images here. One showing the result of Water and the other showing the result of Calf Thymus DNA --->

Justin Landstrom: /* KEY */

Justin Landstrom — Thu, 15 Nov 2012 06:56:43 GMT

KEY

←Older revision		Revision as of 06:56, 15 November 2012
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	* '''Rep''' = Repeat		* '''Rep''' = Repeat
	* '''Drop''' = The values obatined when you draw the best oval around the (green) drop image and then select ANALYZE > MEASURE		* '''Drop''' = The values obatined when you draw the best oval around the (green) drop image and then select ANALYZE > MEASURE
-	* '''Background''' = The values obtained when you draw another oval of the same size in the (green) file for the background above the drop to get the “noise," and select ANALYZE > MEASURE	+	* '''Background''' = The values obtained when you draw another oval of approximately the same size in the (green) file for the background above the drop to get the “noise," and select ANALYZE > MEASURE
	* '''INTDEN''' = Integrated Density		* '''INTDEN''' = Integrated Density
	* '''Integrated Density''' = The sum of the values of the pixels in the selected part of the image. Since the image viewed was the green channel, the value of each pixel is the amount of green present in that pixel. We subtracted the INTDEN from the background of the image in order to eliminate any noise and get a more accurate result.		* '''Integrated Density''' = The sum of the values of the pixels in the selected part of the image. Since the image viewed was the green channel, the value of each pixel is the amount of green present in that pixel. We subtracted the INTDEN from the background of the image in order to eliminate any noise and get a more accurate result.

Justin Landstrom: /* OUR TEAM */

Justin Landstrom — Thu, 15 Nov 2012 06:56:05 GMT

OUR TEAM

←Older revision		Revision as of 06:56, 15 November 2012
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	\|- valign="top"		\|- valign="top"
	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
-	\| [[Image:~~Cosby~~.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]	+	\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
-	\| [[Image:~~yaoming~~.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]	+	\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]
	\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]		\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]
-	~~\| [[Image:BME103student.jpg\|100px\|thumb\|Name: student<br>Role(s)]]~~
	\|}		\|}

Justin Landstrom: /* KEY */

Justin Landstrom — Thu, 15 Nov 2012 06:53:00 GMT

KEY

←Older revision		Revision as of 06:53, 15 November 2012
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	* '''Integrated Density''' = The sum of the values of the pixels in the selected part of the image. Since the image viewed was the green channel, the value of each pixel is the amount of green present in that pixel. We subtracted the INTDEN from the background of the image in order to eliminate any noise and get a more accurate result.		* '''Integrated Density''' = The sum of the values of the pixels in the selected part of the image. Since the image viewed was the green channel, the value of each pixel is the amount of green present in that pixel. We subtracted the INTDEN from the background of the image in order to eliminate any noise and get a more accurate result.
	* '''DNA μg/mL''' = This column of estimated DNA concentrations was calculated using a proportional calibration based on the given values: water's subtracted INTDEN of 3014308 had 0μg/mL and the DNA CALF THYMUS with a subtracted INTDEN value of 3806263 has 2μg/mL of DNA. The specific formula is x = 2.0 * sample INTDEN with background subtracted / calf thymus INTDEN with background subtracted.		* '''DNA μg/mL''' = This column of estimated DNA concentrations was calculated using a proportional calibration based on the given values: water's subtracted INTDEN of 3014308 had 0μg/mL and the DNA CALF THYMUS with a subtracted INTDEN value of 3806263 has 2μg/mL of DNA. The specific formula is x = 2.0 * sample INTDEN with background subtracted / calf thymus INTDEN with background subtracted.
-	* '''Conclusion''' = ~~<!--- explain what "Positive" and "No signal" means, relative to the~~ control samples ~~--->~~	+	* '''Conclusion''' = A sample with a DNA concentration greater than that of our control is therefore positive. After noting that all of the samples were shown to have estimated DNA concentrations much higher than the DNA CALF THYMUS, it is clear that there was most likely a camera positioning error with the DNA CALF THYMUS which resulted in a INTDEn that was far too low.

Justin Landstrom: /* Results */

Justin Landstrom — Thu, 15 Nov 2012 06:50:22 GMT

Results

←Older revision		Revision as of 06:50, 15 November 2012
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	\| '''Sample''' \|\| '''INTDEN (drop)''' \|\| '''INTDEN (background)''' \|\| '''INTDEN with Background Subtracted''' \|\| '''DNA μg/mL''' \|\| '''Conclusion'''		\| '''Sample''' \|\| '''INTDEN (drop)''' \|\| '''INTDEN (background)''' \|\| '''INTDEN with Background Subtracted''' \|\| '''DNA μg/mL''' \|\| '''Conclusion'''
	\|-		\|-
-	\| Water \|\| 3491003 \|\| 466695 \|\| 3014308 \|\| None \|\|	+	\| Water \|\| 3491003 \|\| 466695 \|\| 3014308 \|\| None \|\| --
	\|-		\|-
-	\| Calf Thymus DNA \|\| 4107996 \|\| 301733 \|\| 3806263 \|\| 2.0 \|\|	+	\| Calf Thymus DNA \|\| 4107996 \|\| 301733 \|\| 3806263 \|\| 2.0 \|\| --
	\|-		\|-
	\| PCR: Negative Control \|\| 4240449 \|\| 717685 \|\| 3522764 \|\| 1.9 \|\| Positive		\| PCR: Negative Control \|\| 4240449 \|\| 717685 \|\| 3522764 \|\| 1.9 \|\| Positive

←Older revision		Revision as of 20:39, 15 November 2012
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	\|- valign="top"		\|- valign="top"
	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
-	\| [[Image:~~BME_103_Chiao~~.~~jpg~~\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]	+	\| [[Image:Frontpagepicture.JPG\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
	\| [[Image:jameskyeh.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]		\| [[Image:jameskyeh.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]

←Older revision		Revision as of 20:31, 15 November 2012
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	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
	\| [[Image:BME_103_Chiao.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]		\| [[Image:BME_103_Chiao.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
-	\| [[Image:~~BME_103_Student~~.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]	+	\| [[Image:jameskyeh.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]
	\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]		\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]

←Older revision		Revision as of 20:13, 15 November 2012
Line 9:		Line 9:
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->

-	~~=OUR TEAM=~~	+

	{\| style="wikitable" width="700px"		{\| style="wikitable" width="700px"
	\|- valign="top"		\|- valign="top"
	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
-	\| [[Image:~~BME_103_Student~~.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]	+	\| [[Image:BME_103_Chiao.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
	\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]		\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]

←Older revision		Revision as of 06:56, 15 November 2012
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	\|- valign="top"		\|- valign="top"
	\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]		\| [[Image:BME_103_PersonalPage.jpg\|100px\|thumb\|Name: Justin Landstrom<br>Experimental Protocol Planner and ImageJ Software Processor]]
-	\| [[Image:~~Cosby~~.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]	+	\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: Chiao May Lee<br>Experimental Protocol Planner and Data Compiler and Analyzer]]
-	\| [[Image:~~yaoming~~.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]	+	\| [[Image:BME_103_Student.jpg\|100px\|thumb\|Name: James Kyeh<br>Open PCR Machine Engineer and DNA Measurement Operator]]
	\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]		\| [[Image:XZEGG.jpg\|100px\|thumb\|Name: Jakob G. Wells<br>R&D Scientist]]
	\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]		\| [[Image:Photo.JPGf\|100px\|thumb\|Name: Heidi Hall<br>Open PCR Machine Engineer and Protocol Person]]
-	~~\| [[Image:BME103student.jpg\|100px\|thumb\|Name: student<br>Role(s)]]~~
	\|}		\|}