Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
The old design of the PCR Machine is as follows:
The goal of our PCR redesign is to add more test tube space, making the PCR more efficient:
In order to increase tube space, we increase the heating plate and increase the power proportionally to the size of the plate. We would also add more ventilation next to the heating plate in order to speed up the cooling process of the DNA.
Key Features
Instructions
Protocols
Materials
Supplied in the Kit
Amount
SYBR Green I
def
Calf Thymus DNA
ghi
PCR tubes
95
Negative control
1
Positive control
1
Pipettes
jjk
Microtubes
gjk
GoTAQ Master Mix
jkh
Fluorimeter
jkhjh
Fluroimeter stand and box
lkj
CD with ImageJ software
jhkjh
Supplied by User
Amount
DNA samples
Smartphone for fluorimeter
External computer
Heatproof marker pen
1
PCR Protocol
First, DNA samples were obtained from 31 different patients, each giving 3 DNA samples each. These DNA samples were stored in a freezer in microtubes. For patient number 1, his or her DNA samples were labelled 1a, 1b and 1c, using the heatproof marker. The other DNA samples from the remaining 30 patient were labelled in the same way. After all the DNA samples were obtained, each sample was transferred over to PCR tubes, which are tubes that are used especially for the PCR process. Each PCR tube was labelled in the same way as before. In addition to these 93 PCR tubes, two more tubes, each containing a negative control and a positive control respectively.
DNA Measurement Protocol
Research and Development
Background on Disease Markers
The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 16.
BME 103 Fall 2012
