Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
The old design of the PCR Machine is as follows:
The goal of our PCR redesign is to add more test tube space, making the PCR more efficient:
Key Features
Instructions
Protocols
Materials
Supplied in the Kit
Amount
SYBR Green I
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Calf Thymus DNA
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Pipettes
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Microtubes
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GoTAQ Master Mix
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Fluorimeter
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Fluroimeter stand and box
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CD with ImageJ software
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Supplied by User
Amount
DNA samples
Smartphone for fluorimeter
External computer
Heatproof marker pen
PCR Protocol
DNA Measurement Protocol
Research and Development
Background on Disease Markers
The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 16.