BME103:T130 Group 11 l2: Difference between revisions
Sharon Gooi (talk | contribs) |
|||
Line 135: | Line 135: | ||
'''Background on Disease Markers'''<br> | '''Background on Disease Markers'''<br> | ||
The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 16. | The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 16. Cystic fibrosis gene is a mutation in the gene for the protein cystic fibrosis transmembrane conductance regulator. The cystic fibrosis gene mainly affects the lungs but also the pancreas, liver, and intestines. It is a scarring and cyst formation in the pancreas and causes trouble breathing. This often leads to lung infections. Ultimately death occurs unless a lung transplant can be completed. | ||
More information on the gene can be found at http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35731153 | |||
<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) ---> | <!--- A description of the diseases and their associated SNP's (include the database reference number and web link) ---> | ||
Revision as of 19:45, 27 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
In order to increase tube space, we increase the heating plate and increase the power proportionally to the size of the plate. We would also add more ventilation next to the heating plate in order to speed up the cooling process of the DNA. Key Features
Instructions
ProtocolsMaterials
PCR Protocol
All 95 of the PCR tubes are placed into the PCR machine, and the software for the PCR machine was opened on the computer that the PCR machine was connected to.
Research and DevelopmentBackground on Disease Markers More information on the gene can be found at http://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=35731153
Illustration
|