BME103:T130 Group 11 l2: Difference between revisions
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| Calf Thymus DNA || style="width: 400px;" | ghi | | Calf Thymus DNA || style="width: 400px;" | ghi | ||
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| PCR tubes || style="width: 400 px;" | 95 | |||
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| Negative control || style="width: 400 px;" | 1 | |||
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| Positive control || style="width: 400 pxl" | 1 | |||
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| Pipettes || style="width: 400 px;" | jjk | | Pipettes || style="width: 400 px;" | jjk | ||
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First, DNA samples were obtained from 31 different patients, each giving 3 DNA samples each. These DNA samples were stored in a freezer in microtubes. For patient number 1, his or her DNA samples were labelled 1a, 1b and 1c, using the heatproof marker. The other DNA samples from the remaining 30 patient were labelled in the same way. | First, DNA samples were obtained from 31 different patients, each giving 3 DNA samples each. These DNA samples were stored in a freezer in microtubes. For patient number 1, his or her DNA samples were labelled 1a, 1b and 1c, using the heatproof marker. The other DNA samples from the remaining 30 patient were labelled in the same way. After all the DNA samples were obtained, each sample was transferred over to PCR tubes, which are tubes that are used especially for the PCR process. Each PCR tube was labelled in the same way as before. In addition to these 93 PCR tubes, two more tubes, each containing a negative control and a positive control respectively. | ||
'''DNA Measurement Protocol''' | '''DNA Measurement Protocol''' |
Revision as of 19:34, 27 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
In order to increase tube space, we increase the heating plate and increase the power proportionally to the size of the plate. We would also add more ventilation next to the heating plate in order to speed up the cooling process of the DNA. Key Features
Instructions
ProtocolsMaterials
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Illustration
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