BME103:T130 Group 11 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name:Sharon Gooi Student Role: Experimental Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design The old design of the PCR Machine is as follows: The goal of our PCR redesign is to add more test tube space, making the PCR more efficient: Key Features Instructions Protocols Materials Supplied in the Kit Amount SYBR Green I def Calf Thymus DNA ghi Pipettes jjk Microtubes gjk GoTAQ Master Mix jkh Fluorimeter jkhjh Fluroimeter stand and box lkj CD with ImageJ software jhkjh Supplied by User Amount DNA samples Smartphone for fluorimeter External computer Heatproof marker pen PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers The marker SNP for the disease of cystic fibrosis that is being used is rs35731153. This disease can be found on chromosome 16. Primer Design Illustration