BME103:T130 Group 10 l2

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Also included: Access code for phone app download. <br>
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Also included: Access code for phone app download. <br><br>
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Revision as of 22:06, 28 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
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Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: Frances LakersRole:Protocol Planner
Name:
Frances Lakers
Role:
Protocol Planner
Name: Tyler TamasauckasRole:Protocol Planner
Name:
Tyler Tamasauckas
Role:
Protocol Planner
Name: Jeffery RamirezRole:R&D Specialist
Name:
Jeffery Ramirez
Role:
R&D Specialist
Name: Alexander Baldwin Role:Machine Design
Name:
Alexander Baldwin
Role:
Machine Design

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features

There are two specific features that will be upgraded in the OpenPCR machine - the case and the power source. The case will be changed from a thin wood to a more durable plastic, so that the device can be taken out into the field. The device will also have solar panels, which will provide for the power. This shift allows the device to be used where electricity is not always available, such as rural and undeveloped regions. When combined, these two features open the device up to a larger market.

Instructions

The instructions would remain the same, as the solar panel array would come attached to the plastic on the device's top side.




Protocols

Materials


PCR Protocol
Supplied in Kit

Item Quantity
PCR Machine 1 unit
Camera Phone Stand 1 unit
Flourimeter Stand (LED Light) 1 unit
BlackOut Box 1 unit
Operator's Manual 1 unit

Also included: Access code for phone app download.

Supplied by User

Item Quantity
Smart Phone (w/ Camera) 1 unit
Micropipettes (as needed)
Hydrophobic Glass Slides (as needed)
Eppendorf Tubes Max 16 tubes/trial
Sample DNA 0.2μL/tube; 16 tubes/trial
GoTAQ Master Mix 50μL/tube; 16 tubes/trial
10 μM Forward Primer 1μL/tube; 16 tubes/trial
10 μM Reverse Primer 1μL/tube; 16 tubes/trial
Distilled H2O 47.8μL/tube; 16 tubes/trial
SYBR Green 0.1mL/test sample

DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration


Personal tools