BME103:Logistics: Difference between revisions
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'''MATERIALS & METHODS - DNA AMPLIFICATION''' | '''MATERIALS & METHODS - DNA AMPLIFICATION''' | ||
* Reactions (100 μL total volume) contained: | * Reactions (100 μL total volume) contained: | ||
** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid | ** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127]) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid | ||
** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence. | ** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence. | ||
** 2x GoTaq colorless PCR Master Mix - Promega M7133 | ** 2x GoTaq colorless PCR Master Mix - Promega M7133 |
Latest revision as of 16:12, 2 January 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help |
COURSE SPECS
TEMPLATE PAGES
MATERIALS & METHODS - DNA AMPLIFICATION
MATERIALS & METHODS - DNA IMAGING
OBTAINING MATERIALS
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