BME103:Logistics

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* [[BME103:TEMPwu2 | Lab Write-up 2]]
* [[BME103:TEMPwu2 | Lab Write-up 2]]
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'''MATERIALS & METHODS - DNA AMPLIFICATION'''
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* Reactions (100 μL total volume) contained:
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** Template DNA - Kozak ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176012 BBa_BBa_J176012]) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
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** Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
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** 2x GoTaq colorless PCR Master Mix - Promega M7133
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* A few of the samples were checked via standard ethidium bromide-stained agarose gel electrophoresis outside of class (see [http://openwetware.org/wiki/User:Karmella_Haynes/Notebook/ASU_courses/2012/10/24]). All other samples were measured via SYBRgreen staining on an LED fluorimeter.
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* All reactions were run on an [http://openpcr.org/ Open PCR machine].
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'''MATERIALS & METHODS - DNA IMAGING'''
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* Details to be added soon
'''OBTAINING MATERIALS'''
'''OBTAINING MATERIALS'''

Revision as of 19:10, 2 January 2013

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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COURSE SPECS

  • ~230 Freshmen
    • 3 Lab sections, ~85 students per section
      • 10 - 17 groups, of ~5 students per group
  • 3 hours per lab


TEMPLATE PAGES


MATERIALS & METHODS - DNA AMPLIFICATION

  • Reactions (100 μL total volume) contained:
    • Template DNA - Kozak (BBa_BBa_J176012) inserted in plasmid vector V0120 ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J176127 BBa_J176127) via standard BioBrick ligation; ~10 ng per "positive" reaction; negative reactions contained no plasmid
    • Primers - Forward primer P0001 (5'-gggttttcccagtcacgacg), Reverse primer P0002 (5'-tgtggaattgtgagcggataaca). Amplicon size = 277 bp, spanning some of the vector, the BioBrick prefix sites, Kozak, the BioBrick suffix, and more vector sequence.
    • 2x GoTaq colorless PCR Master Mix - Promega M7133
  • A few of the samples were checked via standard ethidium bromide-stained agarose gel electrophoresis outside of class (see [1]). All other samples were measured via SYBRgreen staining on an LED fluorimeter.
  • All reactions were run on an Open PCR machine.


MATERIALS & METHODS - DNA IMAGING

  • Details to be added soon

OBTAINING MATERIALS

  • We have written several worksheets and hand outs for this course.
  • Please contact one of the instructors if you are interested in obtaining copies.


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