BME103

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
Introduction to Biomedical Engineering - Lab Part 2 Learning Objectives: Students should leave this unit equipped with a theoretical understanding of how to detect DNA biomarkers and the relevance of this technology to human healthcare. Emphasis will be on good lab/experimental controls and the collection of statistically valid measurements. Students will also understand how commonly used lab devices function, and explore the recent efforts to simplify experiments and to lower costs. At the end of the section, students will explore creative new biosensor designs based on PCR and fluorescent imaging. GETTING STARTED Create an OpenWetWare account: Fill in the form at OpenWetWare:How to join Edit your group's wiki: Find your group's Wiki template in one of the Lab Write-Up sections. There are further instructions there. Be creative and have fun editing! THE COURSE IN REVIEW Set-up: OpenPCR Building Before this unit began, a group of ~10 upper level undergrads and graduate students assembled the OpenPCR machines. This was a great experience for the graduate students, and saved our Freshmen engineers the time and trouble of assembling the delicate pieces from scratch in a very limited amount of time. Thanks to our assembly team and Dr. Pizziconi's Design Studio team for your help! Week 1: Introduction - DNA as a Biomarker, 10/17/12 Students were introduced to basic DNA science and its relationship to diagnostics and health. Team members chose roles as Open PCR machine tester, Experimental protocol planner, and Research and development scientist. In concurrent work sessions... Open PCR machine testers partially disassembled, reassembled, and tested the OpenPCR thermal cycler with the guidance of a worksheet and machine manual. Experimental protocol planners planned a Polymerase Chain Reaction (PCR) protocol for the Open PCR system with the guidance of a worksheet. Research and development scientists learned how the Polymerase Chain Reaction works so that they could explain the process to their teammates. This was done with the guidance of the instructor and the OpenPCR Virtual Lab tutorial.