BME100 s2015:Group9 12pmL6: Difference between revisions
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<!-- Instruction 1: Summarize how the consumables (liquid reagents and small plastics) will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | <!-- Instruction 1: Summarize how the consumables (liquid reagents and small plastics) will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | ||
The liquid reagents and mixing fluids will be packaged within their respectable tubes which can be labeled easily with any writing utensil. Both the tips for the micropipettor and tubes will be tinted a charcoal black in order to reduce the affect of light interacting with the liquid reagents like SYBR Green which are light-sensitive. They will all be packaged in a small container that is protective to the shipping and handling. | |||
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<!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | <!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | ||
One major weakness about the use of these consumables is that the SYBR Green I cannot be exposed to light for any reason in order for it not to be contaminated or its concentration make-up to be altered and that limited the amount of time where the liquid reagents can be taken out of the dark and would have limited time managaement and that the experiment would lead to possible errors of the experiment being rushed and under pressure of material limits. | |||
==Feature 2: Hardware - PCR Machine & Fluorimeter== | ==Feature 2: Hardware - PCR Machine & Fluorimeter== |
Revision as of 13:36, 15 April 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||
OUR COMPANYLelouch of Fate Zero Dynasty LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System For this experiment, 68 patients were tested for a disease via a disease-associated SNP. These patients were asked for two samples each of their DNA. The DNA sample pairs were kept together, and a number that was associated with the patient was attached to them. These pairs were distributed amongst 34 teams for testing, amounting to two subjects or four DNA samples per team. The reason for asking each patient for two samples of DNA is to prevent error. That requirement, along with other steps, assures that any anomalies in a single sample will not skew an entire data set. Also, the results from a single patient's samples can be compared to each other and conclusions can be drawn concerning the validity of the test based on the similarity in their results. This is same reasoning behind using multiple drops of DNA solution from each individual sample. Other actions taken to reduce error include positive and negative controls, and the precision of using ImageJ. Adding positive and negative controls established a baseline range for our results, and allowed for direct comparison analysis. This comparison was completed by ImageJ software. The pixel color analysis feature on ImageJ allowed for more accurate, mathematical results as compared to, say, teams visually comparing the color the images side by side. These precautions were necessary to accurately analyze the validity of the chosen test using Bayesian statistics. What Bayes Statistics Imply about This Diagnostic Approach
Computer-Aided DesignTinkerCAD The TinkerCAD tool was used in creating a 3D design of hello Our Design
Feature 1: Consumables KitThe liquid reagents and mixing fluids will be packaged within their respectable tubes which can be labeled easily with any writing utensil. Both the tips for the micropipettor and tubes will be tinted a charcoal black in order to reduce the affect of light interacting with the liquid reagents like SYBR Green which are light-sensitive. They will all be packaged in a small container that is protective to the shipping and handling.
One major weakness about the use of these consumables is that the SYBR Green I cannot be exposed to light for any reason in order for it not to be contaminated or its concentration make-up to be altered and that limited the amount of time where the liquid reagents can be taken out of the dark and would have limited time managaement and that the experiment would lead to possible errors of the experiment being rushed and under pressure of material limits. Feature 2: Hardware - PCR Machine & FluorimeterThe new diagnostic system will have the PCR machine and have included software be integrated into the box that way all of the circuitry and programming will overlap each other with no disturbances or electrical and technical difficulties occurring
during the process. For the software that will be appplicable to the PCR, there will be a design cellphone app that is programmed to only run PCR images on a ImageJ similar app. It will function and run the algorithm automatically when a picture is imported from the phone's camera via high-powered bluetooth technology. Extracting the data will be done with a usb drive. The fluorimeter system will be vastly improved in shape and size where the box will be a safe lock box where one of its sides can attach itself by an interlocking puzzle and be un-attached in order to to put in the whole PCR experiment. The box is also customized with a customized stand for the phone where it will be at a perfect distance of 4 centimeters from the droplets on the fluorimeteer slides. There will be a usb camera clicker that will connect to the phone's camera and be able to take pictures when the box is completely sealed with all of the consumables.
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