BME100 s2015:Group9 12pmL5

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Brianna Chavez Name: Johnathon Ruiz Name: Josh Quick Name: Maria Guzman Name: Taylor Little In US, lab group #1 LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: iPhone 6 Plus Flash: None ISO setting: 800 White Balance: Auto Exposure: Highest Saturation: Lowest Contrast: Highest Calibration After setting up the black box and putting the fluorimeter inside the black box and turning it on, make sure to get a ruler with centimeters and the phone cradle. Then put down the cradle in front of the fluorimeter facing its side and place the phone in the cradle to accurately measure the distance needed.Measure 4 cm from both sides of the cradle in respect of the phone's position to have a close to precise measurement of distance. Distance between the smart phone cradle and drop = 4 cm Solutions Used for Calibration Placing Samples onto the Fluorimeter [Step one, Set the micropipettor to 120 microliters (μL) and put the tip on it. ] [Step two, Obtain 100μL of the DNA sample for ] [Step three, ] [Step four,] [Step five, ] [Step six, ] [Step seven, ] [Step eight, ] [Step nine, ] Data Analysis Representative Images of Negative and Positive Samples Image J Values for All Calibrator Samples TABLE GOES HERE Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP The disease CHD also known as coronary heart disease is a cardiac risk that occurs Primer Design and Testing PCR Product Tube Label AREA Mean Pixel Value RAW INTDEN of the Drop RAWINTDEN of the Background RAWINTDEN Drop- Background row 1 cell 1 Patient 2C row 1 cell 2: 70680 row 1 cell 3: 10.04 row 1 cell 4: 709627.2 row 1 cell 5: 325783 row 1 cell 6: 325783 row 2 cell 1: Patient row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5 row 2 cell 6 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 3 cell 5 row 3 cell 6 row 4 cell 1 row 4 cell 2 row 4 cell 3 row 4 cell 4 row 4 cell 5 row 4 cell 6 row 5 cell 1 row 5 cell 2 row 5 cell 3 row 5 cell 4 row 5 cell 5 row 5 cell 6 row 6 cell 1 row 6 cell 2 row 6 cell 3 row 6 cell 4 row 6 cell 5 row 6 cell 6 row 7 cell 1 row 7 cell 2 row 7 cell 3 row 7 cell 4 row 7 cell 5 row 7 cell 6 row 8 cell 1 row 8 cell 2 row 8 cell 3 row 8 cell 4 row 8 cell 5 row 8 cell 6 row 9 cell 1 row 9 cell 2 row 9 cell 3 row 9 cell 4 row 9 cell 5 row 9 cell 6 row 10 cell 1 row 10 cell 2 row 10 cell 3 row 10 cell 4 row 10 cell 5 row 10 cell 6 row 11 cell 1 row 11 cell 2 row 11 cell 3 row 11 cell 4 row 11 cell 5 row 11 cell 6 row 12 cell 1 row 12 cell 2 row 12 cell 3 row 12 cell 4 row 12 cell 5 row 12 cell 6 row 13 cell 1 row 13 cell 2 row 13 cell 3 row 13 cell 4 row 13 cell 5 row 13 cell 6 row 14 cell 1 row 14 cell 2 row 14 cell 3 row 14 cell 4 row 14 cell 5 row 14 cell 6 row 15 cell 1 row 15 cell 2 row 15 cell 3 row 15 cell 4 row 15 cell 5 row 15 cell 6 row 16 cell 1 row 16 cell 2 row 16 cell 3 row 16 cell 4 row 16 cell 5 row 16 cell 6 row 17 cell 1 row 17 cell 2 row 17 cell 3 row 17 cell 4 row 17 cell 5 row 17 cell 6 row 18 cell 1 row 18 cell 2 row 18 cell 3 row 18 cell 4 row 18 cell 5 row 18 cell 6 row 19 cell 1 row 19 cell 2 row 19 cell 3 row 19 cell 4 row 19 cell 5 row 19 cell 6 row 20 cell 1 row 20 cell 2 row 20 cell 3 row 20 cell 4 row 20 cell 5 row 20 cell 6 row 21 cell 1 row 21 cell 2 row 21 cell 3 row 21 cell 4 row 21 cell 5 row 21 cell 6 row 22 cell 1 row 22 cell 2 row 22 cell 3 row 22 cell 4 row 22 cell 5 row 22 cell 6 row 23 cell 1 row 23 cell 2 row 23 cell 3 row 23 cell 4 row 23 cell 5 row 23 cell 6 row 24 cell 1 row 24 cell 2 row 24 cell 3 row 24 cell 4 row 24 cell 5 row 24 cell 6