OUR TEAM
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[[Image:|100px|thumb|Name: Anthony Gambirazio Role(s)]]
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LAB 4 WRITE-UP
Protocol
Materials
- Lab Coat
- Disposable Gloves
- PCR Reaction Mix, 8 tubes, 50 uL each: Mix contatins Taq DNA polymerase, MgCL2, and dNTP's
- DNA/primer mix, 8 tubes, 50 uL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer
- A strip of empty PCR tubes
- Disposable pipette tips: only use each only once. Never re-use disposable pipette tips or samples will be cross-contaminated
- Cup for discarded tips
- Micropipettor
- OpenPCR machines: shared by two groups
PCR Reaction Sample List
Tube Label |
PCR Reaction Sample |
Patient ID
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G8 P |
Positive control |
none
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G8 N |
Negative control |
none
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G8 1-1 |
Patient 1, replicate 1 |
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G8 1-2 |
Patient 1, replicate 2 |
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G8 1-3 |
Patient 1, replicate 3 |
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G8 2-1 |
Patient 2, replicate 1 |
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G8 2-2 |
Patient 2, replicate 2 |
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G8 2-3 |
Patient 2, replicate 3 |
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DNA Sample Set-up Procedure
- Step 1
- Step 2
- Step 3...
OpenPCR program
Research and Development
PCR - The Underlying Technology
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