BME100 s2015:Group8 9amL4: Difference between revisions
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{| style="wikitable" width="700px" | {| style="wikitable" width="700px" | ||
|- valign="top" | |- valign="top" | ||
| [[Image: | | [[Image:Headshot-BME.jpg|100px|thumb|Name: An Tran<br>Role(s)]] | ||
| [[Image:IMG_1858.jpg|100px|thumb|Name: Molly Golek<br>Role(s)]] | | [[Image:IMG_1858.jpg|100px|thumb|Name: Molly Golek<br>Role(s)]] | ||
| [[Image:IMG_0035.jpg|100px|thumb|Name: Anthony Gambirazio<br>Role(s)]] | | [[Image:IMG_0035.jpg|100px|thumb|Name: Anthony Gambirazio<br>Role(s)]] | ||
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| G8 N || Negative control || none | | G8 N || Negative control || none | ||
|- | |- | ||
| G8 1-1 || Patient 1, replicate 1 || | | G8 1-1 || Patient 1, replicate 1 || 74239 | ||
|- | |- | ||
| G8 1-2 || Patient 1, replicate 2 || | | G8 1-2 || Patient 1, replicate 2 || 74239 | ||
|- | |- | ||
| G8 1-3 || Patient 1, replicate 3 || | | G8 1-3 || Patient 1, replicate 3 || 74239 | ||
|- | |- | ||
| G8 2-1 || Patient 2, replicate 1 || | | G8 2-1 || Patient 2, replicate 1 || 82959 | ||
|- | |- | ||
| G8 2-2 || Patient 2, replicate 2 || | | G8 2-2 || Patient 2, replicate 2 || 82959 | ||
|- | |- | ||
| G8 2-3 || Patient 2, replicate 3 || | | G8 2-3 || Patient 2, replicate 3 || 82959 | ||
|} | |} | ||
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | <!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | ||
# Acquire the DNA samples from the patients as well as PCR reaction mix | |||
# Label all of the PCR tubes for all of the samples that will be tested | |||
# Place a new pipette tip on the micropipette | |||
# Take 50 microliters of the PCR reaction mix | |||
# Transfer PCR reaction mix to the corresponding labeled empty PCR tube | |||
# Discard the used pipette tip | |||
# Put a new pipette tip on the micropipette | |||
# Take 50 micro liters of patient DNA sample/primer corresponding to the label on the tube | |||
# Transfer the patient DNA into the tube containing the PCR reaction mix | |||
# Repeat steps 3 through 9 for all samples. Be sure to place the DNA in the correct labeled tubes | |||
# Place the tubes in the thermal cycler. | |||
'''OpenPCR program''' | '''OpenPCR program''' | ||
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Number of Cycles: 35 | Number of Cycles: 35 | ||
Denature at 95°C for 30 seconds | |||
Anneal at 57°C for 30 seconds | |||
Extend at 72°C for 30 seconds | |||
Final Step: 72°C for 2 minutes | Final Step: 72°C for 2 minutes |
Revision as of 20:16, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
OpenPCR program
Initial Step: 95°C for 2 minutes Number of Cycles: 35 Denature at 95°C for 30 seconds Anneal at 57°C for 30 seconds Extend at 72°C for 30 seconds Final Step: 72°C for 2 minutes Final Hold: 4°C
Research and DevelopmentPCR - The Underlying Technology
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