BME100 s2015:Group8 12pmL6

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR COMPANY Name: Priscilla Hernandez Name: Alwaleed Alsahafi Name: Victoria Sanford Name: Sarah Soaf Name: Taylor Simone Woods LAB 6 WRITE-UP Bayesian Statistics Overview of the Original Diagnosis System What Bayes Statistics Imply about This Diagnostic Approach Computer-Aided Design TinkerCAD Our Design Feature 1: Consumables Kit The consumables kit for the DNA detection lab was not really a kit, since the items were handed to us. For this reason, more time than needed was spent trying to figure out how to start and with what item. Below are lists for the strengths and weaknesses of the kit: Strengths Had everything needed for the experiment Nothing was broken Weaknesses There was no order Chances of breaking something were high The consumables kit will consist of one package containing everything needed for the experiment. The order of the items will be layered, with the first item needed being at the top. This makes it easy for the consumer to take out the items in order. For example, since the first steps of conducting the DNA detection lab is to prepare the tubes, t Micropipettor: This will not necessarily be at the very top, but it will essentially take a whole side of the box because it is the largest item. Tips Tubes Stand Tubes PCR Mix Primers This packaging system will allow for effective use of time, since the all the items will be layered in order. Along with the order of each item, the items will be cushioned with bubble wrap. The box will be line with bubble wrap, as well as in between items to prevent anything from breaking. This is also suitable for the delivery, since the kit can only be ordered online. This kit will be available online on the company website with free standard shipping. Consumers will appreciate that they do not have to go out to look for a place that sells the needed components of DNA detection. The only downside will be that a minimum of one day will be needed for delivery, which is not free. Another weakness may be extreme temperatures of the kit while traveling since it could disrupt the primers and PCR mixes. Feature 2: Hardware - PCR Machine & Fluorimeter A Fluorometer is an instrument that detects fluorescence; quantity is related to the amount of florescent material and, less directly, proportional to the amount of the molecule being detected. By using the fluorimeter method, the ease of having an iPhone to collect data allows for convince. Also it is low cost, because it incorporates a special type of optics, known as an optical caustic, is used. This system is very simple and low cost because there is no need for lasers, mirrors, or lenses. The procedure and instrumentation you used in Lab C is based on the properties of a superhydrophobic surface. The slide is coated with a rough layer of Teflon but has circles of bare glass. This allows the drops to cling to the glass but be repelled by the surface and creates the right environment for two things to happen. Firstly the light from the Blue LED is focused in the drop causing an increase in intensity which helps kick up the fluorescence event, and the SYBR Green I was added with a solvent that helps to form a complex with DNA that prefers to go to the surface. Incorporating both the LED Blue light and SYBR green which are not soluble in water provide the glow in the Fluoermeter box that will be recoded with iPhone. PCR machine have DNA amplifiers that regulate temperature during cyclical programs. PCR machines include things such as lid security, heat block reliability, and Interchangeable blocks to accommodate multi-well plates or tube strips, and programmable scripts. All of these advancements insure more accurate results. The weaknesses for the Fluerometer method was that it was inconvenient having to take on picture at a time, also the stand had to be perfectly level which was a hassle and provided inaccurate results. Also having to transport the images into image J and knowing how to keep track which image is what leaves a lot of room for user error. Weakness of the PCR machine are the fact that it can only do one thing, and that is is to heat up the DNA and cycle it. Although it is effective at what it does, the goal the new device is to add more uses to PCR machine to make it worth the high cost of it.