BME100 s2015:Group7 9amL4: Difference between revisions
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<!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the questions and answers from the Research and Development exercise. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. You are not allowed to use images from current or past BME 100 students' reports on OpenWetWare. --> | <!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the questions and answers from the Research and Development exercise. BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. You are not allowed to use images from current or past BME 100 students' reports on OpenWetWare. --> | ||
The purpose of the PCR (Polymerase Chain Reaction) is to replicate a small strand of DNA sample into a batch large enough to measure through technological means. The DNA sample taken from a patient acts as the template for the replication to base from; meaning after the process of the chain reaction, multiple amounts of the patient's DNA is measurable. While primers in the chemical solution acts as the beginning placeholders for Taq polymerase to | |||
The purpose of the PCR (Polymerase Chain Reaction) is to replicate a small strand of DNA sample into a batch large enough to measure through technological means. The DNA sample taken from a patient acts as the template for the replication to base from; meaning after the process of the chain reaction, multiple amounts of the patient's DNA is measurable. While primers in the chemical solution acts as the beginning placeholders for Taq polymerase to add complementary nucleic acids (such as Adenine, Thymine, Cytosine, Guanine) to the DNA template. The deoxyribonucleotides (dNTP's) acts as the building materials in this solution, for the polymerase to attach onto the separated DNA strand. These are the main components for PCR to perform effectively and efficiently. | |||
Revision as of 19:33, 27 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
The thermal cycling program is a program that is used to control the OpenPCR machine. Using a varied set of input data, the program can tell the machine to heat up to a specific temperature, cool down to a specific temperature, and specify the number of times to repeat the cycle.
Research and DevelopmentPCR - The Underlying Technology
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