OUR TEAM
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LAB 5 WRITE-UP
Procedure
Smart Phone Camera Settings
- Type of Smartphone: IPhone 6
- Flash: Off
- ISO setting: 8
- White Balance: Automatic
- Exposure: Automatic
- Saturation: Automatic
- Contrast: Automatic
Calibration
- Open phone's camera
- Place upright in the cradle provided
- Adjust the distance from phone to the sample making sure the picture is in focus and the distance is greater than 4 cm away.
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- Distance between the smart phone cradle and drop = 4.1 cm
Solutions Used for Calibration
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Placing Samples onto the Fluorimeter
- 1. Pipet 80 milliliters of SYBR Green I solution in the middle of the 2 circles farthest away from the camera.
- 2. Pipet 80 milliliters of calibration solution onto the first drop.
- 3. Make sure the slide is lite by the blue light.
Data Analysis
Representative Images of Negative and Positive Samples
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Image J Values for All Calibrator Samples
Concentration
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Area
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Internal Density
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.25 |
1488 |
69984
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.5 |
1456 |
73079
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1 |
1456 |
72004
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2 |
1321 |
65740
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G1-3 |
Patient1Replicate3 |
73039
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G2-1 |
Patient2Replicate1 |
40429
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G2-2 |
Patient2Replicate2 |
40429
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G2-3 |
Patient2Replicate3 |
40429
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Calibration curve
PCR Results Summary
- Our positive control PCR result was ____ μg/mL
- Our negative control PCR result was ____ μg/mL
Observed results
- Patient _____ :
- Patient _____ :
Conclusions
- Patient _____ :
- Patient _____ :
SNP Information & Primer Design
Background: About the Disease SNP
Primer Design and Testing
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