BME100 s2015:Group6 9amL5

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BME 100 Spring 2015 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Sara Belko
Name: Luc Tieu
Name: Priscilla Delgado
Name: Al Imam
Name: Francisco Campa


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: IPhone 6
    • Flash: Off
    • ISO setting: 8
    • White Balance: Automatic
    • Exposure: Automatic
    • Saturation: Automatic
    • Contrast: Automatic


Calibration

  • Open phone's camera
  • Place upright in the cradle provided
  • Adjust the distance from phone to the sample making sure the picture is in focus and the distance is greater than 4 cm away.
Name: Set Up


  • Distance between the smart phone cradle and drop = 4.1 cm


Solutions Used for Calibration

Name: Water
Name: O.25
Name: 0.5
Name: 1
Name: 2
Name: 5



Placing Samples onto the Fluorimeter 1. Pipet 80 milliliters of SYBR Green I solution in the middle of the 2 circles farthest away from the camera.] 2. Pipet 80 milliliters of calibration solution onto the first drop. 3. Make sure the slide is lite by the blue light.


Data Analysis

Representative Images of Negative and Positive Samples


Image J Values for All Calibrator Samples


TABLE GOES HERE


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing