BME100 s2015:Group6 9amL5: Difference between revisions
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{| style="wikitable" width="300px" | {| style="wikitable" width="300px" | ||
|- valign="top" | |- valign="top" | ||
| [[Image:"setup3".jpg| | | [[Image:"setup3".jpg|500px|thumb|Name: Set Up]] | ||
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{| {{table}} width=700 | {| {{table}} width=700 | ||
|- | |- | ||
| [[Image:"Lab6water".jpg| | | [[Image:"Lab6water".jpg|500px|thumb|Name: Water]] | ||
|- | |- | ||
| [[Image:"Lab60.25".jpg| | | [[Image:"Lab60.25".jpg|500px|thumb|Name: O.25]] | ||
|- | |- | ||
| [[Image:"Lab60.5".jpg| | | [[Image:"Lab60.5".jpg|500px|thumb|Name: 0.5]] | ||
|- | |- | ||
| [[Image:"Lab61".jpg| | | [[Image:"Lab61".jpg|500px|thumb|Name: 1]] | ||
|- | |- | ||
| [[Image:"Lab62".jpg| | | [[Image:"Lab62".jpg|500px|thumb|Name: 2]] | ||
|- | |- | ||
| [[Image:"Lab65".jpg| | | [[Image:"Lab65".jpg|500px|thumb|Name: 5]] | ||
|} | |} | ||
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{| | {| | ||
| align="center" style="background:#f0f0f0;"|'''Concentration''' | | align="center" style="background:#f0f0f0;"|'''Concentration''' | ||
| align="center" style="background:#f0f0f0;"|'''Area''' | | align="center" style="background:#f0f0f0;"|'''Area''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Mean''' | ||
| | | align="center" style="background:#f0f0f0;"|'''RawIntDen Drop''' | ||
| align="center" style="background:#f0f0f0;"|'''Raw IntDen Background''' | |||
| align="center" style="background:#f0f0f0;"|'''Raw IntDen Drop- Background''' | |||
|- | |||
| 0||582||30||17609||2428||15181 | |||
|- | |- | ||
| .25|| | | 0.25||582||39.7||23098||6099||16999 | ||
|- | |- | ||
| .5|| | | 0.5||582||39.6||23071||4984||18087 | ||
|- | |- | ||
| 1|| | | 1||582||26.321||15319||909||14410 | ||
|- | |- | ||
| 2|| | | 2||582||126.3||73506||4821||68685 | ||
|- | |- | ||
| 5|| | | 5||582||112.4||65415||2478||62937 | ||
|- | |- | ||
| | |||
|} | |} | ||
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'''Calibration curve'''<br> | '''Calibration curve'''<br> | ||
<!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. --> | <!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. --> | ||
[[Image:Calibration6.png]] | |||
'''PCR Results Summary''' | '''PCR Results Summary''' | ||
<!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your claculated initial concentration values.--> | <!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your claculated initial concentration values.--> | ||
* Our positive control PCR result was | * Our positive control PCR result was 5 μg/mL | ||
* Our negative control PCR result was | * Our negative control PCR result was 0 μg/mL | ||
<u>Observed results</u> | <u>Observed results</u> | ||
<!-- INSTRUCTIONS: Replace the underscore with each patient ID. After the colon, write both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed --> | <!-- INSTRUCTIONS: Replace the underscore with each patient ID. After the colon, write both a qualitative (what the images looked like) and a quantitative description (μg/mL) of what you observed --> | ||
* Patient | * Patient 73039 : The sample did not contain the allele so it did not glow green. The sample was observed to be 0 μg/mL. | ||
* Patient | * Patient 40429 : The sample did contain the allele so it glowed green. The sample was observed to be 2 μg/mL. | ||
<u>Conclusions</u> | <u>Conclusions</u> | ||
<!-- INSTRUCTIONS: Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. --> | <!-- INSTRUCTIONS: Compare each patient's results to the positive control value and the negative control value. Draw a final conclusion for each patient (positive or negative) and explain why you made that conclusion. --> | ||
* Patient | * Patient 73039 : This patient does not have coronary artery disease. | ||
* Patient | * Patient 40429 : This patient does have coronary artery disease. | ||
<br><br> | <br><br> | ||
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<!-- INSTRUCTIONS: This content is from PCR Lab D. Write a summary, at least five sentences long, about the disease SNP in your own words. --> | <!-- INSTRUCTIONS: This content is from PCR Lab D. Write a summary, at least five sentences long, about the disease SNP in your own words. --> | ||
The disease SNP is associated with lipoprotein lipase, which helps regulate phospholipase activity. It caused by a genetic mutation in the 8th chromosome. More specifically, in the 5' strand, changing AAT to AGT. As a result, it causes coronary artery disease in homo sapiens. Coronary artery disease, or heart disease, is one of the deadliest diseases in America. | |||
'''Primer Design and Testing''' | '''Primer Design and Testing''' | ||
<!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. --> | <!-- INSTRUCTIONS: Write a short summary of the results of your primer test. Underneath your summary, include a screen capture of the results web page. You may crop the image so that it only includes the relevant information. --> | ||
At the end of the primer test, the non-diseased primer test gave a 220bp reading, while the diseased primer had no match. | |||
{| style="wikitable" width="700px" | |||
|- valign="top" | |||
| [[Image:"nondiseased".jpg|500px|thumb|Name: Nondieased]] | |||
| [[Image:"diseased".jpg|500px|thumb|Name: Diseased]] | |||
|} | |||
<!-- Do not edit below this line --> | <!-- Do not edit below this line --> | ||
|} | |} |
Latest revision as of 12:41, 1 April 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPProcedureSmart Phone Camera Settings
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Negative and Positive Samples Image J Values for All Calibrator Samples
Observed results
Conclusions
SNP Information & Primer DesignBackground: About the Disease SNP The disease SNP is associated with lipoprotein lipase, which helps regulate phospholipase activity. It caused by a genetic mutation in the 8th chromosome. More specifically, in the 5' strand, changing AAT to AGT. As a result, it causes coronary artery disease in homo sapiens. Coronary artery disease, or heart disease, is one of the deadliest diseases in America. Primer Design and Testing At the end of the primer test, the non-diseased primer test gave a 220bp reading, while the diseased primer had no match.
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