BME100 s2015:Group5 9amL4: Difference between revisions
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
<!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | <!-- In your own words, write an easy-to-comprehend list of the steps your group took to set up the PCR reaction in the PCR reaction tubes. End with placing the tubes in to the thermal cycler. Do not copy-paste the instructions from the Workbook. That will be considered plagiarism. --> | ||
# Gather all required materials (listed above) | # Gather all required materials (listed above) | ||
# Divide strip of 8 empty tubes into two sets of four | # Divide strip of 8 empty tubes into two sets of four | ||
# Follow the instructions from the Student Workbook and label each of the 8 tubes legibly with a black fine point marker in the following way: | # Follow the instructions from the Student Workbook and label each of the 8 tubes legibly with a black fine point marker in the following way: | ||
<blockquote><OL TYPE="i"> | <blockquote><OL TYPE="i"> | ||
<LI>G (Group) | <LI>G (Group) | ||
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<LI>Patient # (1 or 2) [DASH] Sample # (1, 2, or 3) | <LI>Patient # (1 or 2) [DASH] Sample # (1, 2, or 3) | ||
</OL> | </OL> | ||
((Two of the tubes must be labelled as "control." [P or N])) | ((Two of the tubes must be labelled as "control." [P or N])) | ||
* Patient Example: G5 1-2 (Group 5, Patient 1, Sample 2) | * Patient Example: G5 1-2 (Group 5, Patient 1, Sample 2) | ||
* Control Example: G5 P (Group 5, Positive Control) | * Control Example: G5 P (Group 5, Positive Control) | ||
</blockquote> | </blockquote> | ||
<OL START = "4"> | |||
<LI>Place PCR tubes into the rack. | |||
<LI> | |||
</OL> | |||
'''OpenPCR program''' | '''OpenPCR program''' |
Revision as of 17:44, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
Research and DevelopmentPCR - The Underlying Technology Q1: What is the function of each component of a PCR reaction Template DNA: This is the sequence of DNA that is intended for amplification. It will undergo extreme heat in order for it to denature. Primers: A primer is a single strand of DNA that serves as the starting point for DNA synthesis that binds to specific DNA sequences. Taq Polymerase: This is a thermostable enzyme that recombines nucleotides following separation by heat. It creates a new, complimentary strand of DNA through adding new nucleotides to the old ones. Deoxyribonucleotides (dNTP's): There are the building blocks of DNA: adenine (A), thymine (T), cytosine (C) and guanine (G). These are used by the Taq Polymerase to synthesize the complementary strand of DNA. Q2: What happens to the components (listed above) during each step of thermal cycling?
Q4: During which two steps of thermal cycling does base-pairing occur? Explain your answers.
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