BME100 s2015:Group5 9amL4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 76: | Line 76: | ||
# INITIAL STEP: 95°C for 2 minutes | # INITIAL STEP: 95°C for 2 minutes | ||
# NUMBER OF CYCLES: 35 | # NUMBER OF CYCLES: 35 | ||
<OL TYPE="i"> | <blockquote><OL TYPE="i"> | ||
<LI>Denature at 95°C for 30 seconds | <LI>Denature at 95°C for 30 seconds | ||
<LI>Anneal at 57°C for 30 seconds | <LI>Anneal at 57°C for 30 seconds | ||
<LI>Extend at 72°C for 30 seconds</blockquote> | <LI>Extend at 72°C for 30 seconds | ||
</OL></blockquote> | |||
<OL START = "4"> | <OL START = "4"> | ||
<LI>FINAL STEP: 72°C for 2 minutes | <LI>FINAL STEP: 72°C for 2 minutes |
Revision as of 17:12, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
Research and DevelopmentPCR - The Underlying Technology Q1: What is the function of each component of a PCR reaction Q2: What happens to the components (listed above) during each step of thermal cycling? Q3: DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below?;;;
Q4: During which two steps of thermal cycling does base-pairing occur? Explain your answers.
|