BME100 s2015:Group5 12pmL4: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
=OUR TEAM= | =OUR TEAM= | ||
{| style="wikitable" width="700px" | {| style="wikitable" width="700px" | ||
|- valign="top" | |- valign="top" |
Revision as of 12:34, 25 March 2015
OUR TEAM
Part A
Materials
- Lab coat and disposable gloves
- PCR reaction mix, 8 tubes, 50 μL containing Taq DNA polymerase, MgCl2, and dNTP's
- DNA/primer mix, 50 μl each: all have the same forward and reverse primer but different templates of DNA
- A strip of empty PCR tubes
- Disposable pipette tips: only use each only once. Never re-‐use disposable pipette tips or samples will be cross-‐contaminated
- Cup for discarded tips
- Micropipettor
- OpenPCR machine
DNA Sample Set-up Procedure
- Obtain patient samples, including a positive and negative control, from the TA as well as the patient ID's for group
- Record which ID's went with each patient and label each sample with the ID consistent with the patient
- Do not write the ID's on the tubes, only record on the table
- Add the polymerase, nDTP, and primers to each of the 8 samples
- The samples are prepared for PCR