BME100 s2015:Group4 9amL5
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPProcedureSmart Phone Camera Settings
Calibration
Data AnalysisRepresentative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
Observed results
Conclusions
SNP Information & Primer DesignBackground: About the Disease SNP SNP is a DNA sequence that is associated with coronary heart and artery disease. Primer Design and Testing In the first part of designing the primers, it was necessary to validate the two non disease primers that were designed using the instructions from the lab workbook. The primers tested were as follows. This is the information inputted into the UCSC In-Silico PCR website These are the results of the input. Then in the second part of designing, disease forward and reverse primers were created. The primers tested were as follows: This is the information inputted into the website These are the results of the input From the results, it can be seen that there are no matches to these primers. This is because they are being tested using a non-disease human genome sequence. The disease primers are being compared against a genome that do not have disease.
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