BME100 s2015:Group4 12pmL4

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Sydney Wallace Role(s) File:Nima.Sadeghi.jpg Name: nima.sadeghi Role(s) Jonathan Moroneso Role(s) Name: Fatimah Al habib Role(s) LAB 4 WRITE-UP Protocol Materials Lab Coat Disposable Gloves Micropipettor Disposable Pipet Tips Cup for Pipet Tips PCR Master Mix DNA and Primer Mix Open PCR Machine Ice Bucket Ice PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G# + Positive control none G# - Negative control none G# 1-1 Patient 1, replicate 1 16819 G# 1-2 Patient 1, replicate 2 16819 G# 1-3 Patient 1, replicate 3 16819 G# 2-1 Patient 2, replicate 1 59134 G# 2-2 Patient 2, replicate 2 59134 G# 2-3 Patient 2, replicate 3 59134 DNA Sample Set-up Procedure Put on lab coat and gloves. Prepare ice bucket with ice. Thaw the PCR Master Mix tubes and DNA/Primer mix tubes at room temperature. Vortex and then briefly micro-centrifuge each tube. Prepare each PCR tube with appropriate label as found on the PCR Reaction List Table. Pipet 50μl of Master Mix and 50μl of DNA/Primer mix into each corresponding tube. (Total volume of the third tube is now 100μl.) Immediately place the finished tube into the ice before moving onto the next tube. Place the ready tubes into the thermocycler. OpenPCR program HEATED LID: 100°C INITIAL STEP: 95°C for 2 minutes NUMBER OF CYCLES: 35 Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds FINAL STEP: 72°C for 2 minutes FINAL HOLD: 4°C Research and Development PCR - The Underlying Technology