BME100 s2015:Group2 12pmL5

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Levi Riley Name: Alexandria Clark Name: Blossom Mendonca Name: Alina Kilic Name: Trevor Douglass Name: Andre Dang LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: Iphone 6 Flash: Off ISO setting: Auto White Balance: Auto Exposure: Auto Saturation: Auto Contrast: Auto Calibration Distance between the smart phone cradle and drop = 6.8 cm Solutions Used for Calibration Calibration via H2O Calibration via the standard given which was a concentration 0.25 units of DNA per microliter | Calibration via the standard given which was a concentration 0.5 units of DNA per microliter Calibration via the standard given which was a concentration 1.0 units of DNA per microliter | Calibration via the standard given which was a concentration 2.0 units of DNA per microliter Calibration via the standard given which was a concentration 5.0 units of DNA per microliter | Bonus Picture For the set-up, this picture represents what the set up looked like right before the group closed the flap to take the picture. The camera was set in the cradle and the lens was 6.8 cm away from the drop that was on the superhydrophic side of the glass slide. The box covered the fluorimeter to protect the SyberGreen Dye from exposure to light. After close the flap, three pictures were taken using the timer on the iPhone to delay the taking of the pictures until after the flap was closed. Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Negative and Positive Samples Negative Sample Positive Sample Image J Values for All Calibrator Samples Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP A nucleotide is composed of a nitrogenous base, a five carbon sugar, and at least one phosphate group. They are organic molecules that serve as monomers or sub units of nucleic acids, like DNA and RNA. Polymorphism is variations in coding and non coding DNA sequence among a population. It's a discontinuous genetic variation resulting in the occurrence of several different forms or types of individuals among the members of a single species. The SNP analyzed is found in the Homo Sapien species. The chromosome it is found in is 8:19956018. The clinical significance of the SNP is pathogenic. In addition, it is associated with the LPL (lipoprotein lipase) gene and linked to coronary heart disease. The functions of LPL are Apolipoprotein Binding, Heparin Binding, Lipoprotein Lipase Activity, Phospolipase activity, and Protein Binding. An allele is a variant form of a gene. Some genes have a variety of different forms, which are located at the same position on a chromosome. Disease associated allele is AGT. The numerical position of AGT is 19956018. Primer Design and Testing Diseased Primer <a href="http://imgur.com/ruzkEYS"><img src="http://i.imgur.com/ruzkEYS.png" title="source: imgur.com" /></a> NON-Diseased Primer <a href="http://imgur.com/TAV9kPT"><img src="http://i.imgur.com/TAV9kPT.png" title="source: imgur.com" /></a> The diseased primer had no matches because the humane genome does not have the mutation of AGT. Therefore, the would be no match with the natural human genome tested against in the website. This variation is what makes each individual's makeup unique. The AGT mutation is what makes a person have coronary heart disease.