OUR TEAM
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LAB 5 WRITE-UP
Procedure
Smart Phone Camera Settings
- Type of Smartphone: iPhone
- Flash: Inactivated
- ISO setting: N/A
- White Balance: Auto
- Exposure: N/A
- Saturation: N/A
- Contrast: N/A
Settings that are N/A: Camera is sensitive enough to get a reasonable calibration with DNA concentration
Calibration
- Turn on the camera on the smartphone and calibrate according to the settings above
- Place the smartphone in the cradle
- Place the cradle at a right angle to the slide
- Adjust the height of the fluorimeter using the plastic trays in order to take a picture of the drop sideways
- Adjust the distance between the cradle with the smartphone and the slide so that the cradle is at least 4 cm away from the slide
- Record the distance between the cradle and the slide with a ruler
- Distance between the smart phone cradle and drop = 8cm
Solutions Used for Calibration
row 1 cell 1 |
row 1 cell 2 |
row 1 cell 3 |
row 1 cell 4
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row 2 cell 1 |
row 2 cell 2 |
row 2 cell 3 |
row 2 cell 4
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row 3 cell 1 |
row 3 cell 2 |
row 3 cell 3 |
row 3 cell 4
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- Water at pH = 8
- SYBR Green I Solution
Placing Samples onto the Fluorimeter
- [Instructions: Step one, in your OWN words]
- [Instructions: Step two, in your own words]
- [Instructions: Step three, in your own words]
- [Instructions: Step etc., in your own words]
Data Analysis
Representative Images of Negative and Positive Samples
Positive Sample
Negative Sample
Image J Values for All Calibrator Samples
Negative Sample
Calibration curve
PCR Results Summary
- Our positive control PCR result was ____ μg/mL
- Our negative control PCR result was ____ μg/mL
Observed results
- Patient _____ :
- Patient _____ :
Conclusions
- Patient _____ :
- Patient _____ :
SNP Information & Primer Design
Background: About the Disease SNP
Primer Design and Testing
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