BME100 s2015:Group1 9amL5

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Shawn Striker Name: student Name: student Name: student Name: student Name: student LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: iPhone Flash: Inactivated ISO setting: N/A White Balance: Auto Exposure: N/A Saturation: N/A Contrast: N/A Settings that are N/A: Camera is sensitive enough to get a reasonable calibration with DNA concentration Calibration Turn on the camera on the smartphone and calibrate according to the settings above Place the smartphone in the cradle Place the cradle at a right angle to the slide Adjust the height of the fluorimeter using the plastic trays in order to take a picture of the drop sideways Adjust the distance between the cradle with the smartphone and the slide so that the cradle is at least 4 cm away from the slide Record the distance between the cradle and the slide with a ruler Distance between the smart phone cradle and drop = 8cm Solutions Used for Calibration row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 Water at pH = 8 SYBR Green I Solution Placing Samples onto the Fluorimeter [Instructions: Step one, in your OWN words] [Instructions: Step two, in your own words] [Instructions: Step three, in your own words] [Instructions: Step etc., in your own words] Data Analysis Representative Images of Negative and Positive Samples Positive Sample Negative Sample Image J Values for All Calibrator Samples Negative Sample Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing