BME100 s2015:Group1 9amL5: Difference between revisions

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<!-- INSTRUCTIONS: (1) Show ONE image where you drew a circle around the droplet with the freehand tool for any sample with *no* DNA. (2) Show ONE image where you drew a circle around the droplet with the freehand tool for a sample *with* DNA (positive signal). -If you include more than two images, you will not receive any additional credit. -->
<!-- INSTRUCTIONS: (1) Show ONE image where you drew a circle around the droplet with the freehand tool for any sample with *no* DNA. (2) Show ONE image where you drew a circle around the droplet with the freehand tool for a sample *with* DNA (positive signal). -If you include more than two images, you will not receive any additional credit. -->


 
[[image:positive33.png]]
[[image:negtive33.png]]


'''Image J Values for All Calibrator Samples'''  
'''Image J Values for All Calibrator Samples'''  

Revision as of 09:42, 1 April 2015

BME 100 Spring 2015 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: Shawn Striker
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

Procedure

Smart Phone Camera Settings

  • Type of Smartphone: iPhone
    • Flash: Inactivated
    • ISO setting: N/A
    • White Balance: Auto
    • Exposure: N/A
    • Saturation: N/A
    • Contrast: N/A

Settings that are N/A: Camera is sensitive enough to get a reasonable calibration with DNA concentration


Calibration

  1. Turn on the camera on the smartphone and calibrate according to the settings above.
  2. Place the smartphone in the cradle
  3. Place the cradle at a right angle to the slide
  4. Make sure
  1. Adjust the distance between the cradle with the smartphone and the slide
  • Distance between the smart phone cradle and drop = 8cm


Solutions Used for Calibration

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4



Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Negative and Positive Samples

Image J Values for All Calibrator Samples


Calibration curve


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :




SNP Information & Primer Design

Background: About the Disease SNP


Primer Design and Testing