BME100 s2015:Group1 12pmL5

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Name: student Name: Kaylena Conklin Name: student Name: student Name: student LAB 5 WRITE-UP Procedure Smart Phone Camera Settings Type of Smartphone: IPhone 6 plus Flash: off ISO setting: automatic White Balance: automatic Exposure: automatic Saturation: automatic Contrast: automatic Calibration For this lab, the phone that was used was placed in a horizontal fashion. The camera was on the right side facing the drop of fluid. In an attempt to keep the phone perpendicular to the table, tape was applied to the phone and then attached to the cradle. Earphones were attached to the phone for the purpose of knowing the timing of the pictures being taken. Distance between the smart phone cradle and drop = 7.5 cm Solutions Used for Calibration row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 Placing Samples onto the Fluorimeter Set the micropipette to 80 microliters Collect the CYBER green in the micropipette The light from the Fluorimeter should be shining on the first two rows of dots Place the CYBER green in between the two dots so that the light beam goes straight through the center of the bubble of fluid Eject tip from micropipette and put a new one on While still on the 80 microliter setting, collect the pcr product that has been mixed with the buffer solution Place the pcr solution inside of the CYBER green that is already on the slide Close the box and take pictures Data Analysis Representative Images of Negative and Positive Samples Image J Values for All Calibrator Samples TABLE GOES HERE Calibration curve PCR Results Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ : SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing