BME100 s2015:Group18 12pmL6: Difference between revisions
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==Feature 1: Consumables Kit== | ==Feature 1: Consumables Kit== | ||
<!-- Instruction 1: Summarize how the consumables (liquid reagents and small plastics) will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | <!-- Instruction 1: Summarize how the consumables (liquid reagents and small plastics) will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score. --> | ||
Instead of having to get rid of the tips after every run, we will include a sanitizer to prevent cross-contamination between samples/solutions. We will include an improved micropipette design which includes three switchable/rotatable tubes to switch between different samples so that constant replacement of tips/micropipette is not necessary. This will make the entire process more efficient. | |||
<!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | <!-- Instruction 2: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph. --> | ||
As mentioned previously, our consumables packaging plan will reduce time-wasting and is more cost efficient as resources can be reused more often and less waste is produced as a result of cross-contamination worries. | |||
==Feature 2: Hardware - PCR Machine & Fluorimeter== | ==Feature 2: Hardware - PCR Machine & Fluorimeter== |
Revision as of 14:15, 15 April 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR COMPANY
LAB 6 WRITE-UPBayesian StatisticsOverview of the Original Diagnosis System Error prevention: • Using the black box to cover the sample to protect the fluorescence from interacting with light available in the room • Placing the drop in the middle of the light in order to give the most accurate picture • Dispose the tip of the Micropipette each time to prevent the samples from contamination
Using the data collected from the entire class, we used the following formula P(A|B) = ( P(B|A) * P(A) ) / ( P(B) ) based on the values given to us ( P(A),P(B) and P(B|A) ) for calculation 1 and followed the same procedure for calculations 2, 3 and 4.
• Rounding to the wrong decimal places or rounding off too soon in the calculations • Mixing the results of A and B or negative and positive • Miscounting the POS and negative values • some of data were inconclusive • error might have propagated from wrong data from the different groups
What Bayes Statistics Imply about This Diagnostic Approach
CALCULATION 3:
Final Results:
Computer-Aided DesignTinkerCAD We assembled the original Open PCR machine using TinkerCAD. We placed every part and used the tools to align them and produce the final part using all the pieces. Then, we changed the color to our taste. We decided that the size was an issue because we wanted it to be portable. As such, we made the machine smaller using the transformation tools, and so it is portable. Our Design
We chose this design because we liked the colors; we placed the fans and heating system near each other (near the vents) for better cooling. We put two fans and two heating systems as well as a larger power source to power it more efficiently. The size of the machine was reduced in order to make it portable and convenient.
Feature 1: Consumables KitInstead of having to get rid of the tips after every run, we will include a sanitizer to prevent cross-contamination between samples/solutions. We will include an improved micropipette design which includes three switchable/rotatable tubes to switch between different samples so that constant replacement of tips/micropipette is not necessary. This will make the entire process more efficient.
Feature 2: Hardware - PCR Machine & FluorimeterWe redesigned the PCR machine to include an additional heating unit and fan near the open vents to improve the efficiency of the reactions. We also decreased the size to make the machine more portable. We also added color to the PCR machine make it more aesthetically pleasing and appealing to consumers.
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