BME100 s2015:Group18 12pmL4: Difference between revisions
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NUMBER OF CYCLES: 35 | NUMBER OF CYCLES: 35 | ||
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds | |||
FINAL STEP: 72°C for 2 minutes | FINAL STEP: 72°C for 2 minutes |
Revision as of 20:12, 31 March 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAMLAB 4 WRITE-UPProtocolMaterials
HEATED LID: 100°C INITIAL STEP: 95°C for 2 minutes NUMBER OF CYCLES: 35 Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds FINAL STEP: 72°C for 2 minutes FINAL HOLD: 4°C
Research and DevelopmentPCR - The Underlying Technology Q1. What is the function of each component of a PCR reaction? Q2. What happens to the components (listed above) during each step of thermal cooling? Q3. DNA is made up of four types of molecules called nucleotides, designated as A, T, C and G. Base-pairing, driven by hydrogen bonding, allows base pairs to stick together. Which base anneals to each base listed below? Adenine-Thymine Thymine-Adenine Cytosine-Guanine Guanine-Cytosine
Bonus Round |