DNA/primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer
A strip of empty PCR tubes
Disposable pipette tips: only use each only once. Disposable pipette tips should never be reused or samples will be cross-contaminated
Cup for discarded tips
Micropipettor
OpenPCR machine: shared by two groups
PCR Reaction Sample List
Tube Label
PCR Reaction Sample
Patient ID
G15 +
Positive control
none
G15 -
Negative control
none
G15 1-1
Patient 1, replicate 1
50018
G15 1-2
Patient 1, replicate 2
50018
G15 1-3
Patient 1, replicate 3
50018
G15 2-1
Patient 2, replicate 1
69797
G15 2-2
Patient 2, replicate 2
69797
G15 2-3
Patient 2, replicate 3
69797
DNA Sample Set-up Procedure
Materials mentioned above are obtained from the professors
Two lengths of four tubes should be created by cutting the provided linked tubes in half. Tubes should be empty and will be used for the PCR reaction.
The sides of the tubes should be labeled with a permanent marker then placed in a rack: G15 P (positive control), G15 N (negative control), G15 1-1, G15 1-2, G15 1-3, G15 2-1, G15 2-2, G15 2-3