BME100 s2015:Group13 12pmL5: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
# | # Using the mircopipette, place 80μL of the SYBR GREEN I onto the slide in between the first two rows, then add 80μL of of one of the concentrations of calf thymus from the table on top <br> of the first drop of SYBR GREEN I so that one large drop is formed | ||
# | # Make sure the drop is alighned so that the blue LED light is shining through the large drop | ||
# | # Set the timer on the camera being used, and place the cover on the lightbox to make sure that the picture is taken with little to no light shining through<br> Make sure to take 3 focused images of the drop | ||
# | # Set the micropipette to 160μL and remove the large drop from the slide, before moving the slide to the next position | ||
# Repeat steps 1-4 for the rest of the concentrations from the table | |||
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Revision as of 14:26, 1 April 2015
BME 100 Spring 2015 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Negative and Positive Samples
Image J Values for All Calibrator Samples
Observed results
Conclusions
SNP Information & Primer DesignBackground: About the Disease SNP What is a nucleotide? The organic base units of DNA and RNA What is a polymorphism? A point mutation that is expressed in multiple forms within a population. What species is this variation found in? (latin name) Homo Sapien What chromosome is the variation located on? 8 What is listed as the Clinical significance of this SNP? It is contained within a pathogenic allele Which gene(s) is this SNP associated with? LPL Click the PubMed link to view summaries of research associated with the SNP. What disease is linked to this SNP? Coronary heart disease What does LPL stand for? Lipoprotein Lipase What is the function of LPL? To find out, click the LPL link. Look for “Gene ontology” in the right hand list and click it. Write the first three unique terms you see... apolipoprotein binding heparin binding lipoprotein lipase activity What is an allele? A variation in a gene The disease-associated allele contains what sequence? aat The numerical position of the SNP is 19956018 Non-disease forward primer (20 nt): aatctgggctatgagatcaa The numerical position exactly 200 bases to the right of the disease SNP is: 19956218 Non-disease reverse primer (20 nt): gaaacaccagggctcagggt Disease forward primer (20 nt): aatctgggctatgagatcag Disease reverse primer (20 nt): gaaacaccagggctcagggt Primer Design and Testing
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