BME100 s2015:Group10 12pmL4

BME 100 Spring 2015	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Joseline Valenzuela Experiment Procedure Name: Itai Kreisler Data Analysis Name: Clayton Nunn Role(s) Name: Isaac Clouse Data Analysis) LAB 4 WRITE-UP Protocol Materials Lab coat Disposable gloves PCR reaction mix, 8 tubes, 50 μL each DNA/ primer mix, 8 tubes, 50 μL each A strip of empty PCR tubes Disposable pipette tips Cup for discarded tips micropipettor OpenPCR machine PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G10 + Positive control none G10 - Negative control none G10 1-1 Patient 1, replicate 1 67713 G10 1-2 Patient 1, replicate 2 67713 G10 1-3 Patient 1, replicate 3 67713 G10 2-1 Patient 2, replicate 1 89702 G10 2-2 Patient 2, replicate 2 89702 G10 2-3 Patient 2, replicate 3 89702 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program Research and Development PCR - The Underlying Technology Denaturation: The DNA strands are heated to a high temperature of about 95C. This weakens the hydrogen bonds holding the double helix together, at which point the bonds become so weak, the strands physically separate. The base pairs will be exposed and the DNA is ready for primers to be attached. Annealing: At a lower temperature, of about 55C, the primers are introduced into the mix. Since the DNA base pairs are exposed, the primers will be able to bind to specifically selected sites on the DNA. After this portion, the replication will begin. Extension: DNA polymerase begins to line up the new base pairs at a temperature varying with the polymerase. After a period of time, the DNA will have been doubled. The previous three steps will be repeated amny times in order to amplify a single section of the DNA to be copied millions and millions of times. Final Elongation: The DNA is put through one last extension to ensure all the strands have matching base pairs to form complete double helices. Final Hold: The DNA is stored a cool temperature to prevent it from denaturing again. Credit: http://en.wikipedia.org/wiki/Polymerase_chain_reaction#Procedure