BME100 s2015:Group10 12pmL2: Difference between revisions

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'''Experiment 2''' <br>
'''Experiment 2''' <br>

Revision as of 14:16, 28 January 2015

BME 100 Spring 2015 Home
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OUR TEAM

Name: Joseline Valenzuela Quintero
Name: Clayton Nunn
Name: Your name
Name: Your name
Name: Your name


LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1

Human Study 0mg 5mg 10mg 15mg

Mean(pg/ml) 3.834 8.932 61.622 657.941

StdDev(pg/ml) 1.523010177 1.593931547 30.11069386 212.9429762

Endpoint 10 10 10 10

Std Error(pg/ml) 0.481618106 0.504045412 9.521837451 67.33848166



Experiment 2

Rat Study 0mg 10mg

Mean(pg/ml) 10.516 11.112

StdDev(pg/ml) 2.225551617 7.402885924

Endpoint 5 5

Std Error(pg/ml) 0.995296941 3.310671231





Results

Experiment 1

Experiment 2




Analysis

Experiment 1
Human Test Anova: Single Factor


SUMMARY

Groups Count Sum Average Variance

Column 1 10 38.34 3.834 2.31956

Column 2 10 89.32 8.932 2.540617778

Column 3 10 616.22 61.622 906.6538844

Column 4 10 6579.41 657.941 45344.71112


ANOVA Source of Variation SS df MS F P-value F crit

Between Groups 3027016.695 3 1009005.565 87.25360195 1.40083E-16 2.866265551

Within Groups 416306.0267 36 11564.0563


Total 3443322.721 39

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Experiment 2

T-Test(Rats) - 0.867403497 The p-value was much greater than .05, therefore not statistically significantly different.




Summary/Discussion

In experiment number 1, human test subjects were given different dosages of LPS between 0-15mg in increments of 5mg. There were ten human test subjects per group. The results show that there is a significant difference between the Inflammotin protein levels between the test subjects. This was tested by contrasting the t-test between each possible pair of dosage (i.e: 0-5mg, 0-15mg). We also conducted an ANOVA test on the human results and found again that the results had a significant difference between the Inflammotin protein levels.

In experiment number 2, rat test subjects were given two different dosages of LPS, one group of five rats were given 0mg and the other five rats were given 10mg. The results of the t-test showed that there was not a significant difference of inflammotin protein levels between the two groups.

There was a larger sample size in the humans than the rats which could have altered the results. With a greater sample size of rats we could have had a more accurate representation of the effect on rats. Based on both the ANOVA and the t-test we were able to conclude with statistical certainty that humans had a more reliable positive correlation with LPS than the rats.