BME100 s2014:W Group5 L5: Difference between revisions
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{| | {| | ||
| align="center" style="background:#f0f0f0;"|'''PCR Product TUBE LABEL''' | | align="center" style="background:#f0f0f0;"|'''PCR Product TUBE LABEL''' | ||
| align="center" style="background:#f0f0f0;"|'''Volume | | align="center" style="background:#f0f0f0;"|'''Diluted Volume (µL)''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''SYBR GREEN Volume (µL)''' | ||
| | | align="center" style="background:#f0f0f0;"|'''INTDEN 1''' | ||
| align="center" style="background:#f0f0f0;"|'''INTDEN2''' | |||
| align="center" style="background:#f0f0f0;"|'''INTDEN3''' | |||
| align="center" style="background:#f0f0f0;"|'''AVERAGE INTDENS VALUE''' | |||
|- | |- | ||
| A1||80||80||2716687||3229970||3340085||3095581 | | A1||80||80||2716687||3229970||3340085||3095581 | ||
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| | | | ||
|} | |}'''Fitting a Straight Line'''<br> | ||
'''Fitting a Straight Line'''<br> | |||
''[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]'' | ''[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]'' |
Revision as of 23:07, 15 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
First turn on the Blue LED switch in order to activate the excitation light. Also make sure the camera is open with the settings listed above. From there place the pone on the cradle with 90 degree angle from the slide. Make sure to adjust the height of phone and/or fluorimeter in order to take pictures of the drops. Lastly, the cradle should be as close to the fluorimeter as possible with having a clear picture still.
4.5 cm
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples
[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]
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