BME100 s2014:W Group5 L5: Difference between revisions
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==Data Analysis== | ==Data Analysis== | ||
'''Representative Images of Samples''' | '''Representative Images of Samples'''<br><br> | ||
'''Negative Signal'''<br> | |||
''[[Image:N1.png]]''<br><br> | |||
'''Positive Signal'''<br> | |||
''[ | ''[[Image:P1.png]]''<br> | ||
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''Interpretation''<br><br> | ''Interpretation''<br><br> | ||
Based on the results from the image analysis, the intensity in the green coloration in patient | Based on the results from the image analysis, the intensity in the green coloration in patient B was higher than patient A. Due to the fact that false positives and negatives were controlled for, it can be confidently said that patient B's DNA indicated a positive result for gene detection test. Patient A did not test positive. According to the calibration, it should be noted that the concentration of DNA is positively correlated to the green intensity of the SYBR Green dye; thus the high intensity in patient B's amplified DNA would be expected to increase with increased concentration of DNA. |
Latest revision as of 09:03, 16 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
First turn on the Blue LED switch in order to activate the excitation light. Also make sure the camera is open with the settings listed above. From there place the pone on the cradle with 90 degree angle from the slide. Make sure to adjust the height of phone and/or fluorimeter in order to take pictures of the drops. Lastly, the cradle should be as close to the fluorimeter as possible with having a clear picture still.
4.5 cm
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples
Linear Regression of PCR Results
Interpretation |