BME100 s2014:W Group3 L5: Difference between revisions
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'''PCR Results Summary''' | '''PCR Results Summary''' | ||
''Positive control'' PCR result 2,428,578 μg/mL <br> | |||
''Negative control'' PCR result 935,166 μg/mL <br> | Eight PCR reactions were completed in previous lab. This lab used the SYBR Green I and imaging technique to clearly see the amount of amplified DNA in each reaction. A standard curve converted the INTDEN values to DNA concentration. The threshhold values were measured by the controls of the positive and negative PCR reactions. <br> | ||
''Positive control'' : PCR result 2,428,578 μg/mL <br> | |||
''Negative control'' : PCR result 935,166 μg/mL <br> | |||
''Patient 80926'' : A basic clear shiny drop : Recordings were 325,798 μg/mL, 259,300 μg/mL, and 734,163μg/mL. These numbers are smaller compared to the positive control value and closer in comparison to the negative control. This concludes that the first patient had a negative test result. <br> | ''Patient 80926'' : A basic clear shiny drop : Recordings were 325,798 μg/mL, 259,300 μg/mL, and 734,163μg/mL. These numbers are smaller compared to the positive control value and closer in comparison to the negative control. This concludes that the first patient had a negative test result. <br> | ||
''Patient 83588'' : A bright green fluid drop : Recordings were 3,717,326 μg/mL, 3,488,620 μg/mL, and 2,897,463 μg/mL. These numbers are larger in comparison to the negative control value and closer in comparison to the positive control value. This concludes that the second patient had a positive test result. | ''Patient 83588'' : A bright green fluid drop : Recordings were 3,717,326 μg/mL, 3,488,620 μg/mL, and 2,897,463 μg/mL. These numbers are larger in comparison to the negative control value and closer in comparison to the positive control value. This concludes that the second patient had a positive test result. |
Revision as of 22:04, 15 April 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye SYBR Green Dye is a nucleic acid stain used to identify isolated segments of DNA. Usually utilized to determine whether or not genetic pathogens or discrepancy's are present. Capable of detecting Virus's like HIV as well as precursors for Diabetes. Single-Drop Fluorimeter
ProcedureSmart Phone Camera Settings
Calibration
Solutions Used for Calibration
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] PCR Results Summary
Image J Values for All Samples
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